含氮大环内酯类免疫抑制剂产生菌的系统进化分析  

Phylogenetic Analysis of Nitrogen-Containing Macrolide Immunosuppressant Producing Streptomyces Strains

作　　者：连云阳[1,2] 陈路劼[1] 程元荣[1] 

机构地区：[1]福建省微生物研究所,福建省新药(微生物)筛选重点实验室,福州350007 [2]福建医科大学药学院,福州350004

出　　处：《福建医科大学学报》2009年第5期364-368,共5页Journal of Fujian Medical University

基　　金：福建省自然科学基金(2006J0080)

摘　　要：目的探讨含氮大环内酯免疫抑制剂FK506等不同产生菌之间进化关系及其次级代谢产物合成基因的水平转移。方法克隆不同产生菌的16SrRNA基因以及FK506等代谢产物羟化酶、甲基转移酶基因,采用Mega程序应用不同模型构建菌株系统进化树和合成酶分子进化树。结果代谢产物结构差异越大,其合成酶基因的进化关系越远,但是其产生菌的进化关系却可能较为接近,产生菌的进化关系与代谢产物合成酶分子进化关系不同步。结论此类次级代谢产物合成基因并不是菌株本身固有的,而是在菌株进化过程中同属不同种微生物之间发生基因水平转移获得的。Objective To reveal the evolution relationship of the producers of immunosuppressant FK506, aseomycin and sirolimus and the horizontal gene transfer of their biosynthetic genes. Methods Almost full sequences of the 16S rRNA genes and both cytochrome P-450 hydroxylase and methyltransferase genes involved in the biosynthesis of FK506, ascomycin or sirolimus were cloned from different producers, respectively. Phylogenetic neighbour-joining trees, based on 16S rRNA genes, eytoehrome P-450 hydroxylase or methyhransferase sequences were constructed by Mega 4 programe using different models, respectively. Results Phylogenetic trees showed that the evolution relationship of the two proteins differed from that of the tested five strains. Conclusions The results of inconsistencies between the molecular evolution of biosynthetic enzyme and taxonomic relationships of the producing strains are best explained by horizontal gene transfer,namely, antibiotic biosynthesis genes transfer laterally between Streptomycetes during evolution.

关 键 词：西罗莫司 序列分析 克隆 分子 免疫抑制剂 基因转移 水平 

分 类 号：R96[医药卫生—药理学]