DnaJ-like protein gene sll1384 is involved in phototaxis in Synechocystis sp. PCC 6803  

作　　者：CHEN Zhi XU XuDong 

机构地区：[1]Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China [2]Graduate University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Science Bulletin》2009年第23期4381-4386,共6页

基　　金：Supported by the National Natural Science Fund for Distinguished Young Scientists (Grant No. 30825003)

摘　　要：In Synechocystis sp. PCC 6803, gene sll1384 encodes a protein with a DnaJ domain at its N-terminal portion and a TPR domain at the C-terminal portion. An sll1384 mutant shows no difference from the wild type in adaptation to different temperatures, but almost completely loses its capability of phototactic movement. After complementation with sll1384, the mutant regains the phototaxis. As shown with electron microscopy, on the cell surface, mutant cells have pili that appear to be the same as that of the wild type. Also, the transformation efficiency remains unchanged in the mutant. It is postulated that Sll1384 regulates phototaxis of Synechocystis through protein-protein interaction. It is the first DnaJ-like protein gene identified in a cyanobacterium for a role in phototaxis.In Synechocystis sp. PCC 6803, gene sll1384 encodes a protein with a DnaJ domain at its N-terminal portion and a TPR domain at the C-terminal portion. An sll1384 mutant shows no difference from the wild type in adaptation to different temperatures, but almost completely loses its capability of phototactic movement. After complementation with sll1384, the mutant regains the phototaxis. As shown with electron microscopy, on the cell surface, mutant cells have pill that appear to be the same as that of the wild type. Also, the transformation efficiency remains unchanged in the mutant. It is postulated that Sll1384 regulates phototaxis of Synechocystis through protein-protein interaction. It is the first DnaJ-like protein gene identified in a cyanobacterium for a role in phototaxis.

关 键 词：蛋白基因 趋光性 蛋白质相互作用 程序升温还原 细胞表面 电子显微镜 基因编码 运动能力 

分 类 号：Q51[生物学—生物化学] S852.65[农业科学—基础兽医学]