β_2-AR基因多态性及单倍体型与高血压病并肾功能受损的关联研究  

Relationship between Two β_2-AR Gene Polymorphisms or Haplotypes and Hypertension Complicated by Renal Impairment

作　　者：骆杰伟[1] 陈慧[1] 吴小盈[1] 黄昉萌[1] 孟晓嵘[1] 郑星宇[1] 伍严安[1] 沈晓丽[2] 

机构地区：[1]福建医科大学省立临床医学院,福建省立医院福州350001 [2]福建省心血管病重点实验室,福州350001

出　　处：《中国中西医结合肾病杂志》2009年第11期973-976,I0003,共5页Chinese Journal of Integrated Traditional and Western Nephrology

基　　金：福建省卫生厅中医药科研基金资助项目(No.WZY0626);福建省自然基金资助项目(No.2008J0322)

摘　　要：目的:探讨β2-AR基因多态性与高血压病(EH)并肾功能受损易感性的关系。方法:收集158例EH并肾功能受损患者(EH-RI组),及与之按性别、年龄、居住地相匹配的肾功能正常的EH无肾功能受损组(EH-non-RI组)及血压肾功能正常对照组各158例。应用PCR-RFLP检测β2-AR(-1023G/A,rs2053044;+252G/A,rs1042717)多态性。用SHEsis网络软件估计单倍体型频率。结果:χ2分割显示:EH-RI组β2-AR/-1023的AA频率(13.29%)高于EH-non-RI组(6.33%)、正常组(4.43%)(χ2=8.940,P<0.01)。Logistic回归分析,以EH-non-RI组为参照系,调整危险混杂因素后,β2-AR/-1023-AA型患者EH-RI的OR值为2.561(P<0.05)。在构建的单倍体型中,EH-RI组的G-G单倍体型(β2-AR/-1023—β2-AR/+252)频率(40.19%)低于EH组(47.78%)、正常组(48.10%)(χ2=5.109,P<0.05)。结论:携带有β2-AR/-1023-AA基因型是患EH-RI易感因素之一,G-G单倍体型(β2-AR/-1023—β2-AR/+252)对EH-RI有保护作用。Objective：To investigate the association between hypertension complicated by renal impairment（RI） and the two variants of the β2-adrenoreceptor gene in Chinese.Methods：EH-RI group of 158 subjects were collected by evaluating serum creatinine level,and matched by subjects of EH-non-RI group with normal renal function and control subjects for individual age,gender and geography.All the subjects were genotyped for the polymorphisms of β2-AR/-1023,β2-AR/＋252,and the genetic variants frequencies were compared in all subjects.The SHEsis program was employed to estimate the haplotype frequencies.The SPSS program was used to perform the logistic regression analysis.Results：Partitions of χ2 method showed that the higher prevalence of β2 -AR/-1023-AA genotypes was noted in EH-RI group（χ2=8.940,P0.05）.And logistic-regression indicated that β2-AR/-1023-AA genotypes was a risk factor for EH-RI with the reference category of EH-non-RI.The OR of the β2-AR/-1023-AA genotypes vs GA/GG for EH-RI was 2.561 （95%CI：1.102～5.592,P0.05） after adjusting for risk factors of EH-RI.The G-G haplotype （β2-AR/-1023—β2 -AR/＋252） was significantly less frequent in EH-RI individuals than that in EH-non-RI group and in control （Partitions of χ2 method,χ2=5.109,P0.05）.Conclusion：The β2-AR/-1023-AA genotypes is significantly associated with EH-RI,and G-G haplotype（β2-AR/-1023—β2-AR/＋252） may contribute to be a preventive factor to EH-RI.

关 键 词：高血压 肾功能受损 基因 多态现象 单核苷酸 Β2肾上腺素受体 

分 类 号：R544.1[医药卫生—心血管疾病]