小鼠限食/低温游泳模型评价黄连、吴茱萸及其复方寒热药性  

COLD and HOT nature of Coptis & Evodia and their prescriptions investigated with diet restriction/cold-water swimming mice models

作　　者：任永申[1,2] 王伽伯[1] 赵艳玲[1] 张萍[1] 赵海平[1,2] 张学儒[1] 周灿平[1] 肖小河[1] 金城[1] 

机构地区：[1]解放军中药研究所解放军302医院,北京100039 [2]成都中医药大学药学院,四川成都610075

出　　处：《药学学报》2009年第11期1221-1227,共7页Acta Pharmaceutica Sinica

基　　金：国家重点基础研究发展计划(973计划)资助项目(2007CB512607;2006CB504703)

摘　　要：建立黄连、吴茱萸及其复方左金丸、反左金丸寒热药性评价的新方法。通过限食游泳、饲喂高蛋白饲料复制小鼠体质模型;采用冷热板示差法,考察药物对小鼠温度趋向性的干预作用;同时测定耗氧量、肝组织ATP酶活性,考察药物对能量代谢的影响。结果表明,药物作用按"黄连→左金丸→反左金丸→吴茱萸"方向传递;即黄连增加动物在高温区分布,降低耗氧量及Na+-K+-ATP酶活性(n=6,P<0.01或P<0.05),而吴茱萸表现相反;提示黄连之性"寒"与吴茱萸之性"热",与传统理论认识一致。该方法可以直观且客观地表征黄连、吴茱萸寒热药性的差异及配伍后的变化趋势,为中药药性理论研究提供了新的技术手段。To establish a new method to evaluate the COLD and HOT nature of Coptis Evodia and their prescriptions Zuojinwan and Fanzuojinwan. Physical models of mice were established by diet restriction with cold-water swimming （weak model, WM） and fed with high protein animal feeds （strong model, SM）. An instrument with cold and hot pads was used to investigate the variation of temperature tropism among SM and WM groups of mice affected by drugs. Meanwhile, the oxygen consumption and activity of adenosine triphosphatase （ATPase） were detected, in order to investigate the mechanism of energy metabolism which might be affected by these drugs. The results showed that the drug effects gradually changed in an order of ＂Coptis → Zuojinwan → Fanzuojinwan → Evodia＂. In detail, Coptis increased the remaining rate （RR） of mice on hot pad, decreased oxygen consumption and ATPase activity （n = 6, P〈0.01 or P〈0.05）, while Evodia performed inversely; which indicated the CLOD nature of Coptis and HOT nature of Evodia, and confirmed with their traditional definition in medicinal works. In conclusion, the methods applied in this work, can objectively and directly express the nature disparity between the two herbs and predict the tendency of changes of the nature of their combination, which brings a new approach in investigation of the nature theory of traditional Chinese medicine.

关 键 词：中药寒热药性 动物行为学 温度趋向性 黄连 吴茱萸 

分 类 号：R285.1[医药卫生—中药学]