8-羟基二氢小檗碱改善高FFA和高糖诱导的3T3-L1脂肪细胞胰岛素抵抗的作用  

8-Hydroxy-dihydroberberine ameliorated insulin resistance induced by high FFA and high glucose in 3T3-L1 adipocytes

作　　者：徐丽君[1] 陆付耳[1] 易屏[2] 王增四[1] 魏世超[3] 陈广[2] 董慧[1] 邹欣[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院中西医结合研究所,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院中医科,湖北武汉430030 [3]华中科技大学同济医学院附属同济医院药学部,湖北武汉430030

出　　处：《药学学报》2009年第11期1304-1308,共5页Acta Pharmaceutica Sinica

摘　　要：我国应用小檗碱临床治疗2型糖尿病已有30多年的经验,虽确有疗效,但其药效强度不够,始终不能成为治疗糖尿病的主导药物。小檗碱的口服吸收率太低是其降糖效果不佳的主要原因。研究者对小檗碱的结构进行了化学修饰，The purpose of the study is to investigate the effect of 8-hydroxy-dihydroberberine on insulin resistance induced by high free fatty acid （FFA） and high glucose in 3T3-L1 adipocytes and its possible molecular mechanism. Palmic acid or glucose in combination with insulin was used to induce insulin resistance in 3T3-L1 adipocytes. 8-Hydroxy-dihydroberberine and berberine were added to the cultured medium separately, which were considered as treated group and positive control group. The rate of glucose uptake was determined by 2-deoxy-[3H]-D-glucose method. The amount of glucose consumption in the medium was measured by glucose oxidase method. Cell growth and proliferation of 3T3-L1 adipocytes were detected with Cell Counting Kit-8 （CCK-8） assay. After incubated with palmic acid for 24 hours or glucose with insulin for 18 hours, the rate of glucose transport in 3T3-L1 adipocytes was inhibited by 67% and 58%, respectively. The amount of glucose consumption in 3T3-L1 adipose cells was decreased by 41% after cells were incubated with palmic acid for 24 h. However, the above changes were reversed by pretreatment with 8-hydroxy-dihydroberberine for 24 and 48 h. Significant difference existed between groups. Insulin resistance in 3T3-L1 adipocytes, which is induced by high FFA and high glucose, could be ameliorated by 8-hydroxy-dihydroberberine.

关 键 词：8-羟基二氢小檗碱 胰岛素抵抗 葡萄糖 游离脂肪酸 

分 类 号：R965[医药卫生—药理学]