内皮细胞Rho/Rho激酶介导纤维肉瘤细胞血管外游走的机制  

Mechanism of Endothelial Rho/Rho Kinase in Extravascular Migration of Fibrosarcoma Cell

作　　者：辛华[1] 高忠礼[2] 韩振国[1] 

机构地区：[1]吉林大学中日联谊医院胸外科,长春130033 [2]吉林大学中日联谊医院骨外科,长春130033

出　　处：《中国医学科学院学报》2009年第5期594-597,I0003,共5页Acta Academiae Medicinae Sinicae

基　　金：吉林省杰出青年科技支持计划(20070156)~~

摘　　要：目的研究内皮细胞Rho/Rho激酶在纤维肉瘤细胞血管外游走过程中的调控机制。方法采用血管外游走体外模型观察纤维肉瘤细胞血管外游走状况;测定游走过程中血管内皮单细胞层电阻变化;进行细胞内磷酸化实验,利用放射自显影技术评价纤维肉瘤细胞游走过程中内皮细胞肌球蛋白轻链磷酸化水平。结果纤维肉瘤细胞可以穿过血管内皮细胞游走至血管外,在游走过程中跨血管内皮细胞层电阻下降,内皮细胞肌球蛋白轻链磷酸化水平升高。内皮细胞Rho抑制剂(C3转移酶)、Rho激酶抑制剂(Y-27632)能够抑制上述变化。结论内皮细胞Rho/Rho激酶可能是通过调节肌球蛋白轻链磷酸化水平诱导纤维肉瘤细胞向血管外游走。Objective To study the mechanism of endothelial Rho/Rho kinase in extravascular migration of fibrosarcoma cell. Methods We used an in vitro model of fibrosarcoma cell transmigration across a mono- layer of human umbilical vein endothelial cell（HUVEC）cultured on collagen gel to observe extravascular migration of fibrosarcoms cells, and then calculated the electrical resistance of HUVEC monolayer and endothelial myosin light chain （MLC） phosphorylation in extravascular migration of fibrosarcoma cells. Results Fibrosarcoma cells migrated through endothelial cells into collagen gel. The electrical resistance of a HUVEC monolayer reduced and endothelial MLC phosphorylation enhanced in the extravascular migration of fibrosarcoma cells. Endo- thelial Rho inhibitor （ C3 transferase） and Rho kinase inhibitor （Y-27632） inhibited the extravascular migration of fibrosarcoma cells and inhibited the reduction of electrical resistance of a HUVEC monolayer and the enhancement of endothelial MLC phosphorylation in extravascular migration of fibrosarcoma cells. Conclusion Endothelial Rho/Rho kinase may regulate fibrosarcoma cell transendothelial migration through MLC phosphorylation.

关 键 词：纤维肉瘤细胞 血管外游走 血管内皮细胞 RHO/RHO激酶 

分 类 号：R725.970.2[医药卫生—儿科] R73-33[医药卫生—临床医学]