雀麦上雀麦腥黑粉菌Tilletia bromi的分子检测  

Molecular detection of Tilletia bromi on brome grasses

作　　者：廖芳[1,2] 赵磊[1] 罗加凤[2] 黄国明[2] 刘跃庭[2] 李明刚[1] 

机构地区：[1]南开大学生命科学学院,天津300071 [2]天津出入境检验检疫局,天津300456

出　　处：《菌物学报》2009年第6期776-782,共7页Mycosystema

基　　金：国家十一五科技支撑计划(No.2006BAK10B06);国家863项目(No.2007AA06Z323);国家质检总局课题(No.2008IK236);天津市科技支撑项目(No.06YFGZNC00700)

摘　　要：雀麦是重要的牧草,近年来进口量激增。寄生于雀麦上的腥黑粉菌共有4种,即小麦矮腥黑穗菌Tilletia controversa(TCK)、雀麦腥黑粉菌T.bromi、T.bolayi和小麦网腥黑穗菌T.caries(TCT),根据冬孢子形态难以直接区分这些种类。本文在形态、自发荧光和萌发生理三方面的比较研究基础上,依据beta-微管蛋白tub2基因序列设计一套引物,转化为特异SCAR分子标记,建立了雀麦上T.bromi的菌丝基因组DNA的特异PCR检测方法和冬孢子的套式特异PCR检测方法,为病害提供了快速、可靠的检测方法。Brome grasses are economically and ecologically important forage grasses, and have been frequently introduced into China these years. The smut fungi Tilletia spp. are important pathogens on these grasses and have greatly endangered grass production. There are four Tilletia species, i.e. T. controversa （TCK）, T. bromi, T. bolayi and T. caries （TCT）, infecting these grasses. It is difficult to separate them from each other based on the characteristics of their teliospores. In this study, the morphology, autofluorescence and germination behavior of teliospores were determined and four species, T. bromi, TCK, TCT and T. fusca with similar characteristics were selected for further analysis. Based on the nucleotide difference of a beta-tubulin tub2 gene, a molecular marker that is sequence characterized amplified region （SCAR） was developed specifically for PCR detection of T. bromi by using the DNA samples from either the mycelia or the teliospores isolated from brome grasses. The method developed in this study can be quickly and reliably employed in quarantine analysis.

关 键 词：SCAR分子标记 套式PCR 分子检测 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]