双孢蘑菇耐热相关基因的表达载体构建及转化研究  

The expression vector construction and transformation of thermotolerance-related gene of Agaricus bisporus

作　　者：陈美元[1] 廖剑华[1] 郭仲杰[1] 李洪荣[1] 卢政辉[1] 蔡丹凤[1] 王泽生[1] 

机构地区：[1]福建省农科院食用菌研究所福建省蘑菇菌种研究推广站,福州350014

出　　处：《菌物学报》2009年第6期797-801,共5页Mycosystema

基　　金：国家科技支撑计划课题(No.2007BAD89B13)

摘　　要：构建了双孢蘑菇Agaricus bisporus耐热相关基因028-1全长cDNA序列的双元表达载体,通过农杆菌介导转化双孢蘑菇非耐热菌株8213,经潮霉素抗性筛选和PCR鉴定,获得了一批双孢蘑菇转基因菌株。对10株转基因菌株进行了不同温度下的草管走菌试验,结果显示大部分转基因菌株的耐热性能有较为明显的提高。A binary expression vector of thermotolerance-related gene 028-1 ofAgaricus bisporus was constructed and transferred into non-thermotolerance strain 8213 of A. bisporus by Agrobacterium-mediated transformation. After hygromycin resistance selection and PCR identification, dozens of transgenic strains of A. bisporus were obtained. 10 of them were put into compost running test under different temperatures, and the results showed that the thermotolerance of most of the transgenic strains increase obviously.

关 键 词：食用菌 耐热性状 转基因 潮霉素抗性 农杆菌 

分 类 号：Q943.2[生物学—植物学]