微紫青霉菌Penicillium janthinellum菌株GXCR吸附废水中Cd^(2+)的研究  

Cd^(2+) biosorption in wastewater by Penicillium janthinellum strain GXCR

作　　者：黄晓婷[1,2] 马义丽[1,2] 李有志[1,2] 

机构地区：[1]广西大学生命科学与技术学院,南宁530005 [2]微生物及植物遗传工程教育部重点实验室,南宁530005

出　　处：《菌物学报》2009年第6期850-856,共7页Mycosystema

基　　金：广西科技攻关项目(No.0895003-8&No.0443001-20);广西亚热带生物资源保护利用重点实验室主任基金(No.07-04);微生物与植物遗传工程教育部重点实验室开放基金(No.J0701)

摘　　要：研究了5种不同预处理方式对丝状真菌微紫青霉菌Penicillium janthinellum菌株GXCR的Cd2+吸附的影响。结果表明,高温(80℃)、去离子水中的匀浆化、匀浆+碱化(NaOH,0.5mol/L)(简称匀浆碱化)和匀浆+30%二甲基亚砜处理均能提高菌体的吸附率,其中匀浆碱化处理后菌体的吸附效果最佳,吸附增量达到117.96%;匀浆+酸化(H2SO4,0.5mol/L)处理则导致菌体的Cd2+吸附能力显著下降。匀浆碱化菌体吸附符合典型的Langmuir方程,表明该菌对Cd2+的吸附可能是以表面吸附为主的吸附行为。在吸附-解吸附循环4次后匀浆碱化菌体的Cd2+的吸附效率为58.01%。红外光谱分析显示匀浆碱化处理主要影响菌体表面分子的–OH和C=O基团,其中与Cd2+结合的主要基团是–OH。结果也表明,匀浆碱化菌体具有处理电镀废水的潜能。Effects of five pretreatment methods on Cd2＋ biosorption of the mycelia of Penicillium janthinellum strain GXCR were investigated. The result showed that pretreatments of oven dry at 80℃, homogenate of mycelia in deionized water, basified homogenate of mycelia （NaOH, 0.5mol/L） and homogenate of mycelia in C2H6SO （30%） solution could enhance Cd2＋ biosorption efficiency, in which pretreatment of basified homogenate was most effective, increasing the Cd2＋ biosorption by 117.96%; acidified homogenate of mycelia in H2SO4 （0.5mol/L） led to significant decrease in Cd2＋ bioadsorption capacity. Biosorption of basifiedhomogenate of mycelia followed Langmuir isotherm equation, suggesting that the Cd2＋ biosorption is surface sorption. After four sorption-desorption cycles, the basified homogenate of mycelia still had a Cd2＋ biosorption efficiency of 58.01%. Infrared spectrometry analysis indicated that basified homogenate pretreatment might affect radicals of –OH and C=O on the mycelial surfaces, and the principal radicals linking Cd2＋ was –OH. The results also indicated that basified homogenate of the mycelia might have a potential for the treatment of electroplating wastewater.

关 键 词：真菌 预处理 生物吸附 解吸附 吸附机理 电镀废水 

分 类 号：X703[环境科学与工程—环境工程]