巨细胞病毒DNA在不同血管组织的分布及其与动脉粥样硬化的关系  

Distribution of cytomegalovirus DNA in vascular tissues and the relationship between virus and atherosclersogenesis

作　　者：程远[1] 李多多[2] 程新 刘宝玲 程计林[1] 

机构地区：[1](复旦大学附属)公共卫生临床中心,上海市201508 [2]河南省新乡医学院第一附属医院小儿内一科 [3]Worcest Polytechnic Institute,USA [4]Massachusetts General Hospital, Harvard Medical School, Harvard University, USA

出　　处：《中华实验和临床病毒学杂志》2009年第5期321-324,共4页Chinese Journal of Experimental and Clinical Virology

基　　金：复旦大学上海医学院基础.临床交叉研究基金（KSF0174）;上海市（复旦大学附属）公共卫生临床中心人才引进科研启动基金（RCJJP7） 本课题曾得到美国哈佛大学哈佛医学院Crumpacker教授、Jielin Zhang助理教授、金壮、王海斌、刘玉珍研究员的帮助和指导,数据的统计学处理得到复旦大学附属上海市公共卫生临床中心周晓明教授的指导,在此一并感谢.

摘　　要：目的探讨巨细胞病毒（CMV）感染后病毒在动、静脉血管组织中的分布及其与动脉粥样硬化发生的关系。方法（1）通过腹腔注射病毒建立C57BL／6J小鼠巨细胞病毒感染模型；（2）感染12周后取颈动脉、主动脉、心脏和后腔静脉进行病理学检查，并用PCR检测是否其组织中存在CMVDNA；（3）EusA方法检测血清中的白细胞介素6（IL-6）和单核细胞趋化蛋白-1（MCP-1）。结果（1）病毒感染组6只鼠中2只出现典型的主动脉粥样硬化病理学改变；未感染病毒对照组6只鼠未出现任何病理变化；（2）病毒感染组6只鼠的主动脉和后腔静脉均发现CMV DNA，4只鼠的颈动脉和心肌组织中也存在CMVDNA。未感染病毒组6只鼠血管组织中未发现CMVDNA；（3）病毒感染组鼠的血清中IL广6（25％与75％值间中位值113．7pg／ml）和MCP。1（128．7pg／ml）明显高于对照组（49．77pg／ml和45．36pg／ml，P〈0．05）。结论心血管内皮细胞是CMV潜伏的场所；CMV感染及与其感染有关的细胞因子增加促进动脉粥样硬化发生的病理过程。Objective To explore the distribution of cytomegalovirus （CMV） in vascular tissues and the relationship between virus and atherosclerogenesis after CMV infecting mice. Methods （ 1 ） C57 BL/6J Murine model of CMV infection was established by intraperitoneal injection of CMV lethiferous amount. （2） After 12 weeks of CMV infection, the sera, carotids, aorta, hearts and postcaval veins from the mice were collected under euthanasia. The tissues would be used to DNA extraction, PCR and pathological examination. （3）Interleukin-6 （IL-6） and monocyte chemotactic protein-1 （MCP-1） in serum were measured with ILELISA. Results （1）The typical pathologic feature in 2 aorta samples of 6 mice infected by CMV was found and the mice uninfected by CMV did not show any pathologic change. （2）CMV DNA appeared in 6 aorta, 6 postcaval veins, 4 carotids and 4 heart tissues including endocardium, cardiac muscle and coronary artery from the CMV infected mice. CMV DNA was not found in the vascular and heart tissues from 6 mice uninfected by CMV. （3） The ELISA test showed the significant difference（ Mann-Witney test of Nonparametric Test, P 〈 0.05 ） in serum IL-6（ Median among 25 % and 75 % percentile ： 113.7pg/ml vs 49.77 pg/ml） and MCP- 1 （ Median among 25 % and 75 % percentile ： 128.7pg/ml vs45.36 pg/ml）between CMV infected mice and uninfected mice. Conclusion Cardiovascular cells are CMV latent reservoir in host body and CMV infection and the cytokines induced by CMV infection probably relate to atherosclerogenesis.

关 键 词：巨细胞病毒 动脉硬化 细胞因子类 

分 类 号：R543[医药卫生—心血管疾病]