乙型肝炎病毒假病毒体外感染树鼩原代肝细胞模型的建立  

Hepatitis B Virus Pseudoparticles Can Infect Primary Tupaia Hepatocytes Specifically In Vitro

作　　者：王学军[1] 田琳琳[1] 杨静[1] 周喆[1] 丁晓然[1] 苏婧[1] 李丽红[2] 王升启[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]中国科学院昆明动物研究所灵长类研究中心,云南昆明650223

出　　处：《生物技术通讯》2009年第6期753-756,共4页Letters in Biotechnology

基　　金：国家自然科学基金重点项目(30530650);国家重点基础研究发展计划(2005CB522902);国家杰出青年基金(30625041)

摘　　要：目的:建立乙型肝炎病毒假病毒(HBVpp)体外感染树鼩原代肝细胞(PTH)模型。方法:通过肝脏原位两步灌注法分离PTH并对其冻存方法进行优化,通过共转染293T细胞生产基于慢病毒包装系统的HBVpp并考察其感染的种属和组织特异性。结果:通过肝脏原位两步灌注法分离了PTH,并优化了PTH冻存液的配方;包装的HBVpp具有与HBV真病毒类似的感染种属和组织特异性。结论:该模型的建立对深化HBV进入肝细胞机制的研究具有重要意义。Objective： To establish an in vitro infection cell model of hepatitis B virus pseudoparticles（HBVpp） based on primary tupaia hepatocytes（PTH）. Methods： PTH were prepared by two-step collagenase perfusion and three different cryopreservation formulations were compared. HBVpp were produced by cotransfection 293T cells with lentiviral packaging system and their species and tissue specific infection ability was analyzed. Results： PTH were isolated successfully and the cryopreservation method was optimized. HBVpp were produced and they could infect PTH specifically in vitro. Conclusion： The establishment of HBVpp-PTH in vitro infection eell model would promote the HBV entry mechanism research.

关 键 词：乙型肝炎病毒 假病毒 树鼩原代肝细胞 体外感染细胞模型 

分 类 号：R373[医药卫生—病原生物学] R512.62[医药卫生—基础医学]