反相柱层析制备去乙酰真菌环氧乙酯  

Preparation of Deacetylmycoepoxydiene with Reversed Phase Chromatography

作　　者：周显凤[1] 张伟[1] 黄耀坚[1] 郑忠辉[1] 沈月毛[1] 

机构地区：[1]厦门大学生命科学学院

出　　处：《厦门大学学报（自然科学版）》2009年第6期871-874,共4页Journal of Xiamen University：Natural Science

基　　金：国家海洋863项目(2007AA091503)资助

摘　　要：研究不同条件下反相柱层析对去乙酰真菌环氧乙酯分离纯化的影响.通过RP-18中压柱层析对从红树林内生真菌拟茎点霉(Phomopsissp.)A-1-2-3诱变后突变株G1-1固体发酵提取物中的去乙酰真菌环氧乙酯进行了纯化精制.对RP-18柱层析纯化去乙酰真菌环氧乙酯操作条件进行了考察,使去乙酰真菌环氧乙酯的含量从28%左右提高到81.5%左右,回收率大于90%.采用RP-18直接洗脱,溶析结晶,重结晶,结晶残液RP-18分步洗脱的总工艺路线,去乙酰真菌环氧乙酯的总回收率为87.7%,产品最终纯度达到99%以上.The effects on purification of deaeetylmycoepoxydiene（DAM） in different conditions were studied. Preparation of DAM from the crude extract of fermentation of strain G1-1, a mutant strain of mangrove endophytic phomopsis sp. A-1-2-3 ,was conducted using Cls-silica gel reversed phase chromatography at moderate pressure. In the process, the operating conditions of Cas-silica gel chromatography were studied experimentally, then the purity of DAM reached about 81.5 % from about 28 % in the crude extract, and the recovery of DAM was over 90%. The whole process of preparation was as follow： direct elution with RP-18, solvent-out crystallization, recrystallization and segment elution of residue of crystallization with RP-18. Through the whole process flow, the general recovery of DAM was 87.7%, and the purity of DAM reached 99% or higher.

关 键 词：拟茎点霉 去乙酰真菌环氧乙酯 柱层析 分离纯化 

分 类 号：Q93[生物学—微生物学]