HRAP基因诱导型植物表达载体的构建及烟草转化  

Construction of Inducible Plant Expression Vector of HRAP Gene and Transform Tobacco

作　　者：王爱英[1] 缪建锟[1] 彭晓明[1] 沈海涛[1] 祝建波[1] 

机构地区：[1]石河子大学生命科学学院,石河子大学农业生物技术重点实验室,石河子832003

出　　处：《西北农业学报》2009年第6期157-160,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：国家自然基金(30860071);国家转基因重大专项项目(2009ZX08011-002);石河子大学高层次人才启动项目;石河子大学科学研究发展计划“动植物育种专项计划项目”(gxjs2007-yz13)

摘　　要：系统获得性抗性是植物抵御病菌侵染的一种独特的防御机制。HRAP基因是一种从甜胡椒中克隆的蛋白质激发子,可以有效引发植物系统性抗性。SAR8.2b基因是烟草系统获得性抗性信号传导途径中下游响应基因,受病原物和水杨酸诱导。本试验根据Genebank发布SAR8.2b基因的启动子序列,利用PCR技术,克隆了SAR8.2b基因的完整启动子,命名为SAR8.2bP,然后将SAR8.2bP构建到pUC18-HRAP载体中命名为pUC18-SAR8.2bP-HRAP,然后用SAR8.2bP-HRAP替换p2300-121载体中的35S启动子和GUS基因,重组子命名p2300-SAR8.2bP-HRAP。将构建的植物表达载体转化农杆菌GV3101,利用叶盘法转化烟草,通过PCR筛选,获得了转基因植株。为进一步验证转基因烟草的抗病效果奠定了基础。In the co-evolution with pathogens,plants have evolved complex integrated defense mechanisms against pathogens.Systemic acquired resistance is an important component of plant disease resistance mechanisms.HRAP is a elicitor which cloned from sweet pepper.It could trig plant SAR efficiently.SAR8.2b was cloned gene was at downstream of tobacco SAR transduction pathway.The promoter of SAR8.2b,which could inducible by SA and pathogens.The SAR8.2bP was constructed to pUC18-HRAP,then replaced the 35S promoter and GUS by SAR8.2bP-HRAP.The inducible plant expression vector was named for p2300-SAR8.2bP-HRAP.The recombine vector was transferred into Gv3101.The tobacco was transformed by leaf-disc method.Transgenic plants were identified by PCR.All of this will make the foundation of plant anti-diseases.

关 键 词：HRAP基因 SAR8.2b启动子 系统获得性抗性 转基因烟草 

分 类 号：Q943.2[生物学—植物学] S572[农业科学—烟草工业]