农杆菌Ri诱导丹参毛状根培养体系的优化  

Establishment of Hairy Roots Culture System of Salvia

作　　者：刘晓艳[1] 王渭玲[1] 李学俊[1] 

机构地区：[1]西北农林科技大学,陕西杨凌712100

出　　处：《西北农业学报》2009年第6期183-186,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金："十一五"国家支撑计划项目(2007BAD79B01)资助

摘　　要：采用发根农杆菌R1601菌株浸染丹参叶片、茎段、叶柄、带节茎段等外植体。结果表明,相比茎和叶柄,丹参叶片更适宜诱导毛状根;带节茎段经发根农杆菌R1601侵染后,幼苗根系较为发达,粗壮。而由其他3种丹参外植体诱导产生的毛状根生长相对较慢,形成的根较细而弱。Aimming to optimizing Salvia miltiorrhiza hairy root culture system.Explant of leaves,stem,petiole were used to introduce via Agrobacterium rhizogenes R1601 strain in this study,The result showed that compared with stem and petiole,leaves of S.miltiorrhiza are more suitable for hairy root induction;Stem section with nod infected with Agrobacterium rhizogenes transforms seedlings,the transformed S.miltiorrhiza.Plant shows large root system,fibrous-like,and internode reducing significantly.This research provide the theory and technology for the establishment of efficient genetic transformation system of Salvia miltiorrhiza Bunge.

关 键 词：发根农杆菌 毛状根 丹参 培养体系 再生植株 

分 类 号：S567.53[农业科学—中草药栽培]