转基因仙客来的生根移栽及耐热性  

Techniques of Rooting and Transplantation and Heat-tolerande Identification of Transformed Cyclamen

作　　者：刘倩[1] 梁东[1] 马锋旺[1] 

机构地区：[1]西北农林科技大学园艺学院,陕西杨凌712100

出　　处：《西北农业学报》2009年第6期268-271,共4页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：西北农林科技大学"拔尖人才支持计划"项目

摘　　要：以转Mn-SOD基因仙客来植株为材料,筛选出仙客来块茎诱导的不定芽生根培养基,研究仙客来组培苗的移栽技术及高温逆境胁迫对转基因和未转基因植株的生理机制的影响。研究表明:仙客来不定芽最适生根培养基为1/2MS+1.0 mg/L IBA;在38℃高温胁迫下,转基因仙客来的SOD活性高于未转基因植株,细胞膜透性和MDA的含量上升的速度均低于未转基因植株,可溶性蛋白含量高于未转基因植株,初步表明转基因植株的耐热性高于未转基因植株。The objective of this study was to research about Cyclamen persicum,Mn-SOD gene was incorporated into plants.Screening out the ideal rooting medium of adventitious bud regeneration through tube.Researched on the techniques of transplantation of Cyclamen and also researched on physiological changes of high temperature stress of transformed plantlets and non-transformed plantlets,expecting for evaluate its effect on the endurance to high temperature stress and environmental stress,pave the way for its breeding via genetic engineering.The main results were as follows：Screening out the best rooting medium of Cyclamen is 1/2MS＋1.0 mg/L IBA.Study on high temperature stress tolerance in Mn-SOD gene transformed Cyclamen plantlets.Physiological changes of high temperature stress at 38℃ of transformed and non-transformed Cyclamen was measured.The experimental data showed that the level of SOD activity in transformed Cyclamen was higher than non-transformed plantlets;the membrane permeability and MDA content in transformed Cyclamen rose more slowly than non-transformed plantlets;the protein content in transformed Cyclamen was higher than non-transformed plantlets.These results indicated that the tolerance of the transformed plantlets to high temperature stress was higher than non-transformed control.

关 键 词：仙客来 高温胁迫 生根移栽 生理指标 

分 类 号：S682.262[农业科学—观赏园艺]