发状念珠藻不同细胞破碎方法的研究  

Comparison of Different Cell Disruption Methods for Nostoc Flagelliforme

作　　者：丁文杰[1] 岳思君[1] 郭伟[1] 秦韶燕[1] 贾士儒[1] 

机构地区：[1]天津科技大学工业微生物教育部重点实验室,天津300457

出　　处：《现代食品科技》2009年第11期1243-1245,1261,共4页Modern Food Science and Technology

基　　金：国家自然科学基金资助项目(No.20776112);国家高技术研究发展计划(863)资助项目(No.2008AA10Z326);国家重点基础研究发展计划(973)资助项目(No.2007CB714305)

摘　　要：为了获得野生发状念珠藻和液态培养发状念珠藻细胞破碎和提取藻蓝蛋白的最佳方法,进行了发状念珠藻细胞破碎方法的研究。采用液氮研磨、反复冻融,超声破碎,高压均质和玻璃珠破碎这几种方法对野生和液态培养发状念珠藻进行破碎研究。通过测定破碎率、藻蓝蛋白提取得率和纯度,对几种细胞破碎效果和藻蓝蛋白提取方法进行比较。结果表明,采用高压均质方法能获得最佳的破碎效果,当野生发状念珠藻和液态培养发状念珠藻细胞密度为10mg/mL时,破碎率分别达到96.45%和97.06%。反复冻融法为提取液态培养发状念珠藻藻蓝蛋白最理想的方法,其藻蓝蛋白的纯度和提取得率分别为0.468和1.28%;高压均质为提取藻蓝蛋白的最理想方法,其藻蓝蛋白纯度和提取得率分别为0.153和1.43%。In order to get the best method for extraction of C-phycocyanin from Nostocflagelliforme, the cell disruption of N.flagelliforme were investigated using five methods, including liquid nitrogen grounding, repeated freezing and melting, ultrasonic disruption, high-pressure homogenization, and glass beads-breaking treatment. And the five methods were compared by determination the breakage rate, extraction rate and purity of C-phycoeyanin. Results showed that high-pressure homogenization was the most efficient method for cell disruption, with which the breakage rates of wild and liquid-cultured Nostoeflagelliforme reached 96.45% and 97.06%, respectively, when their cell density was 10 mg/mL. And repeated freezing and melting method was the most suitable method for extraction of C-phycocyanin from liquid-cultured Nostoc flagelliforme, with which the extraction rate and purity of C-phycocyanin were 1,28% and 0.468, respectively. For extraction of C-phyeoeyanin from wild Nostocflagelliforme, high-pressure homogenization showed to be the best one. Using this method, the extraction rate and purity of C-phycocyauin were 1.43% and 0.153, respectively.

关 键 词：藻蓝蛋白 细胞破碎 发状念珠藻 

分 类 号：Q94-3[生物学—植物学]