PAMAM-SiO2复合纳米颗粒增强的葡萄糖传感器  

Research of PAMAM-SiO_2 nano-particles strengthened glucose biosensor

作　　者：连兰[1] 伍林在 易德莲[2] 秦晓蓉[2] 黄峰[2] 

机构地区：[1]吉林师范大学化学学院,四平136000 [2]武汉科技大学应用化学研究所,武汉430081

出　　处：《传感器世界》2009年第11期16-19,38,共5页Sensor World

基　　金：湖北省自然科学基金资助(编号:2003ABA075);湖北省教育厅自然科学基金资助(编号:2003A011)

摘　　要：将PAMAM-SiO2复合纳米颗粒和葡萄糖氧化酶通过壳聚糖-SiO2溶胶-凝胶固定于普鲁士蓝修饰的工作电极表面,然后以自制铂片电极为对电极、甘汞电极为参比电极,组成三电极体系,测定电镀废水中重金属离子的浓度。考察了电极的选择、葡萄糖氧化酶的固定量、复合颗粒的修饰量、温度、缓冲溶液的pH值、扫描速率等因素对葡萄糖传感器响应信号的影响。实验结果表明:选用铂盘电极、葡萄糖氧化酶固定量为4.9 U、添加浓度为1.5 mmol/L纳米复合颗粒3.5μL、在25℃、pH 6.64、扫描速率为20 mV/s的条件下进行测量,响应电流最大。检测Hg^(2+)时,传感器的线性响应范围为2.5～22.5 μmol/L,检出限为2.5×10^(-6)mol/L,相关系数为0.97(n=9)。In this paper, chitosan and SiO2 sol-gel are taken as cross-linking reagents, and PAMAM-SiO2 nanoparticles and glucose oxidase（GOD） are immobilized onto the Prussian blue/platinum electrode. The three-electrode system which is composed of two platinum electrodes and saturated calomel electrode acting as working electrode, counter electrode and reference electrode respectively, executes a glucose biosensor and is used to detect the concentration of heavy metal ions in plating wastewater. The choices of electrode, concentration of GOD and nanoparticles, temperature, pH value of solution and scan rate are studied emphatically. The results show, the 4.9 U GOD and 3.5 μL of 1.5 mmol/L nanoparticles in pH 6.64 solution at the temperature 25℃ are appropriate conditions using platinum electrode, and the scan rate is 20 mV/s. The linear range of the biosensor is 2.5-22.5 μmol/L when Hg2＋ is detected. The detection limit of the biosensor is 2.5× 10^-6 mol/L, and the correlation coefficients is 0.97 （n=9） .

关 键 词：葡萄糖氧化酶 纳米二氧化硅 重金属离子 树形大分子 生物传感器 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]