O-硬脂酰化壳聚糖作为DNA的递送载体材料  

O-stearoyl-chitosan as DNA delivery vectors

作　　者：苏均乐[1] 徐瑾[1] 吕玉明[2] 谢德明[1] 

机构地区：[1]暨南大学生物医学工程系,广东广州510630 [2]广州医学院附属第三医院,广东广州510200

出　　处：《暨南大学学报（自然科学与医学版）》2009年第5期548-552,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：教育部生物医用高分子材料重点实验室(武汉大学)资助;广东省自然科学基金项目(07002690);广东省医学科学科技研究基金项目(WSTJJ20061103)

摘　　要：以邻苯二甲酰基预先保护壳聚糖分子中-NH2,以硬脂酰氯进行可控酯化,制备O-硬脂酰化壳聚糖.通过超声振荡与溶剂结合制备O-硬脂酰化壳聚糖/DNA复合物,采用动态激光散射及原子力显微镜分析复合物的粒径分布及形态.凝胶电泳考察该载体与DNA的结合能力.L929细胞培养对O-硬脂酰化壳聚糖进行细胞毒性评估.FT-IR和1HNMR结果表明,可以成功制备不同酯化度的O-硬脂酰壳聚糖;O-硬脂酰化壳聚糖与DNA结合可形成不规则球形复合物,平均粒径可达到86nm,并且对DNase显示高稳定性.同时O-硬脂酰化壳聚糖具有促L929细胞增殖能力,安全性较高,可作为一种新型的非病毒型基因载体.Using phthalic anhydride to selectively protect the -NH2 groups of chitosan molecules, the O-stearoyl-chitosan compounds were successfully prepared with different esterification degree by changing the ratios of stearoyl chloride and chitosan in the reactions. O-stearoyl-chitosan /λ DNA complexes were harvested by sonieation technology combined with organic solvents. Laser scatter analysis and atom force microscopy were used to give the results of O-stearoyl-chitosan /λ DNA complexes particles size and distribution, particle morphology respectively. The conjunctive ability of O-stearoyl-chitosan with λ DNA was evaluated by agarose gel electrophoresis analysis, and the cellular toxicity experiment was examined by MTT using L929 cells. FT-IR and ^1H NMR results demonstrated the formation of different esterification degree of modified chitosan. The shape of the complexes were irregular sphere, and the particles could reach 86 nm and have high stability under DNase conditions. The O-stearoyl-chitosan could make L929 cells proliferation as well. So, O-stearoyl-chitosan will become a new effective non-virus DNA carrier.

关 键 词：O-硬脂酰化壳聚糖 载体 基因递送 

分 类 号：R943.3[医药卫生—药剂学]