腹泻性贝毒在翡翠贻贝中的积累、排出及其对HSP70表达的影响  

Accumulation of DSP toxins and HSP70 expression in Perna viridis exposed to Prorocentrum lima

作　　者：商兴芳[1] 杨维东[1,2] 刘洁生[1,2] 张剑军[1] 李宏业[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632 [2]水体富营养化与赤潮防治广东省高等学校科研型重点实验室,广东广州510632

出　　处：《暨南大学学报（自然科学与医学版）》2009年第5期571-575,共5页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(40976065);国家自然科学基金-广东省联合基金重点项目(U0733006);广东省自然科学基金项目(031885)

摘　　要：以利玛原甲藻(Prorocentrum lima)为饵料,通过室内滤食实验,考查了腹泻性贝毒(diarrheic shellfishpoisoning,DSP)在翡翠贻贝(Perna viridis)体内的累积与排出规律,分析了HSP70基因的表达与毒素累积与排出之间的关系.结果显示,翡翠贻贝暴露于产毒利玛原甲藻后,其滤食率显著下降,存在明显的剂量-效应关系.毒素积累阶段,翡翠贻贝鳃组织中DSP毒素显著增加,均明显高于对照组(P<0.05).停止染毒后,贻贝体内毒素含量显著下降.染毒后,随着毒素的累积,HSP70显著增加,并于第2天达到高峰.这些结果提示,翡翠贻贝对DSP毒素有一定的清除及耐受能力,HSP70基因的诱导表达可能是贻贝对DSP毒素胁迫的一种适应性反应,可能在贻贝抗DSP毒素中发挥重要作用.In order to explore the roles of HSP70 gene in resistant mechanism of shellfish to diarrheic shellfish poisoning （DSP） toxins, the accumulation and cleaning action of DSP toxins in Perna viridis were studied, and HSP70 gene expression induced by DSP toxins were detected, too. The results indicated that the grazing rates of P. viridis decreased significantly in a dose-effect pattern. The level of DSP toxins in gills of P. viridis increased significantly in a dose-dependent relation with time. However, the toxin levels in P. viridis descended significantly after withdrawal of P. lima. HSP70 expression increased obviously in a dose-dependent relation, and achieved the highest at the 2nd day after exposed to DSP. These results suggested that P. viridis had some resistant ability to DSP toxins, and HSP70 gene expression might have an important role in the resistant mechanism.

关 键 词：翡翠贻贝 腹泻性贝毒 热激蛋白70 利玛原甲藻 

分 类 号：X55[环境科学与工程—环境工程] S823.2[农业科学—畜牧学]