长沙地区肺炎克雷伯菌质粒介导AmpCβ内酰胺酶的检测与分析  

Prevalence of plasmid-mediated AmpC beta-lactamases in the Klebsiella pneumoniae strains isolated in Changsha

作　　者：张运丽[1] 祝林[1] 刘文恩[1] 

机构地区：[1]中南大学湘雅医院检验科,湖南长沙410008

出　　处：《中国感染与化疗杂志》2009年第6期454-457,共4页Chinese Journal of Infection and Chemotherapy

基　　金：湖南省自然科学基金资助项目(06JJ4104)

摘　　要：目的研究湖南长沙地区肺炎克雷伯菌中质粒介导AmpCβ内酰胺酶的检出率和基因型。方法收集2007年10月—2008年7月湖南长沙地区中南大学3所附属医院临床分离的非重复肺炎克雷伯菌110株,采用头孢西丁纸片扩散法进行肺炎克雷伯菌AmpC酶表型初筛,采用多重PCR法测定AmpC酶表型阳性肺炎克雷伯菌的ampC耐药基因型。结果在110株临床分离肺炎克雷伯菌中24株细菌对头孢西丁纸片不敏感,经多重PCR扩增21株菌在约405bp处出现阳性条带,特异性PCR显示此21株菌均携带DHA型ampC耐药基因。产质粒介导AmpC酶肺炎克雷伯菌的检出率为19.1%(21/110)。结论长沙地区临床分离的肺炎克雷伯菌株中质粒介导产AmpC酶肺炎克雷伯菌检出率高,其质粒介导的ampC耐药基因全为DHA基因型。Objective To study the plasmid-mediated AmpC β-lactamases and the genotypes of K. pneumoniae in Changsha region. Methods A total of 110 non-duplicate strains of K. pneumoniae were isolated from October 2007 to July 2008 in the three affiliated hospitals of Central South University in Changsha, Hunan province. Cefoxitin disk diffusion method was used to screen the K. pneumoniae for phenotypic AmpC enzyme. Multiple PCR method was adopted to test ampC genotypes of the AmpC enzyme-positive K. pneumoniae strains. Results Of all the 110 clinical isolates of K. pneumoniae, 24 were not sensitive to cefoxitin disk, 21 showed positive 405 bp bands by multiple PCR. Specific PCR confirmed that the 21 strains carried DHA type arnpC gene. The prevalence of plasmid-mediated AmpC enzyme in K. pneurnoniae was 19. 1% （21/110）. Conclusions The prevalence of plasmid-mediated AmpC enzyme in K. pneumoniae isolates is relatively high in Changsha area. The identified plasmid-mediated ampC genes are all DHA genotype.

关 键 词：肺炎克雷伯菌 AMPC酶 质粒 

分 类 号：R446.5[医药卫生—诊断学] R450[医药卫生—临床医学]