一株高效脱硫菌的分离鉴定和脱硫特性研究  

Isolation,identification and desulfurization characteristics of an efficient desulfurization strain

作　　者：贡俊[1,2] 张肇铭[1] 王玉芬[2] 杨官娥[3] 郑军[1] 程红兵[1] 李保珍[1] 

机构地区：[1]山西大学生命科学与技术学院,太原030006 [2]山西财经大学环境经济学院,太原030006 [3]山西医科大学药学院,太原030001

出　　处：《环境工程学报》2009年第11期2031-2036,共6页Chinese Journal of Environmental Engineering

基　　金：国家科技攻关计划项目(2001BA540C);国家自然科学基金资助项目(30470269)

摘　　要：从污水净化厂二沉池回流污泥中分离到一株硫杆菌菌株d j-5,该菌株是一种兼性厌氧菌,通过生理生化特性鉴定,并结合16S rDNA序列分析及鉴定,该菌株可以确定为脱氮硫杆菌（Thiobacillus denitrificans）。该菌株的生长曲线表明菌体生长迅速,延滞期约为8 h,然后细菌进入对数生长期,这一阶段持续时间大约20～24 h,稳定期较短,细菌很快进入衰亡期。通过脱硫工艺实验考察了pH值、温度和搅拌速度对脱硫率和菌体生长的影响,结果表明,在进气量180 L/h、pH=6～8、温度为25～35℃和搅拌速度为270 r/min时,该菌株能正常生长,对进气浓度高达2 500 mg/m^3的硫化氢脱除率在91%以上。A strain dj-5 of Thiobacillus was isolated from the reflux sludge in secondary sedimentation tank of a sewage treatment plant.The strain was facultative and anaerobic.According to the analysis of physiological and biochemical measurements and 16S rDNA gene sequence,the strain was identified with Thiobacillus denitrificans.The growth curve indicated that the strain grew rapidly and initiate retardation phase of the strain were 8 h and then became logistic phase which sustained 20-24 h after that entered stabilization phase which was short and finally entered failed phase.The effects of pH,temperature and agitation rate on H2S removal efficiency and the strain were studied.The results suggested that the strain grew well and the H2S removal efficiency was more than 91% under concentration 2 500 mg/m^3,gas flow 180 L/h,pH 6～8,temperature 25～35℃ and agitation rate 270 r/min.

关 键 词：脱氮硫杆菌 16SrDNA 鉴定 硫化氢 

分 类 号：X701.3[环境科学与工程—环境工程]