川芎菌核病的症状及病原菌鉴定  

Symptoms of sclerotinia stem rot on Ligusticum chuanxiong and Identification of its pathogen

作　　者：罗玲[1] 黄云[1] 王靖[1] 何苗[1] 万英[1] 

机构地区：[1]四川农业大学植物病理学系,雅安625014

出　　处：《植物病理学报》2009年第5期532-535,共4页Acta Phytopathologica Sinica

基　　金：四川省科技厅攻关招标项目(2006Z08-091)

摘　　要：Symptoms of sclerotinia stem rot of Ligusticum chuanxiong were observed and the causal agent of the disease was identified.The results showed that the symptoms of this disease began with the formation of dark brown hygrophanous splotch on the basal leaves,followed by the appearance of dark brown circular spot on the basal stem.Finally,the whole plant stoped growing and died.White mycelium and/or sclerotia were formed under moisturized conditions.On PDA medium,the pathogen of this disease formed brown sclerotia with various shapes and 2-5 mm in diameter.The diameter of apothecia was 0.3-0.8 cm and the length of stipes was 2-8 cm.Asci were columniform in shape and(100-135)×9 μm in size,each containing 8 ascospores sized by(6-12) μm×(3-7) μm.No asexual spore formation was observed.Based on the morphological characteristics and the sequence of ribosomal DNA-ITS,the pathogen was identified as Sclerotinia sclerotiorum(Lib.) de Bary.Symptoms of sclerotinia stem rot of Ligusticum chuanxiong were observed and the causal agent of the disease was identified. The results showed that the symptoms of this disease began with the formation of dark brown hygrophanous splotch on the basal leaves, followed by the appearance of dark brown circular spot on the basal stem. Finally, the whole plant stoped growing and died. White mycelium and/or sclerotia were formed under moisturized conditions. On PDA medium, the pathogen of this disease formed brown sclerotia with various shapes and 2 - 5 mm in diameter. The diameter of apothecia was 0.3 - 0.8 cm and the length of stipes was 2-8 cm. Asci were columniform in shape and （100 -135）×9μm in size, each containing 8 ascospores sized by （6 - 12 ） μm×（ 3 - 7 ）μm. No asexual spore formation was observed. Based on the morphological characteristics and the sequence of ribosomal DNAITS, the pathogen was identified as Sclero- tinia sclerotiorum （Lib.） de Bary.

关 键 词：病原菌鉴定 川芎 症状 中药材 四川省 伞形科 根茎 止痛 

分 类 号：S436.8[农业科学—农业昆虫与害虫防治] S567.239[农业科学—植物保护]