苹果轮纹病菌诱导产孢方法  

Method to promote sporulation of Botryosphaeria berengeriana f.sp.Piricola

作　　者：冷伟锋[1] 李保华[1] 国立耘[2] 董娟华[1] 王彩霞[1] 李桂舫[1] 董向丽[1] 

机构地区：[1]青岛农业大学植物保护学院,山东青岛266109 [2]中国农业大学植物病理系,北京100193

出　　处：《植物病理学报》2009年第5期536-539,共4页Acta Phytopathologica Sinica

基　　金：公益性行业(农业)科研专项(nyhyzx07-055)

摘　　要：In order to get a great quantity of spores of Botryosphaeria berengeriana f.sp.piricola,several methods to promote sporulation of the pathogen were compared.The results showed that the wounded young apple fruits within 60-day old was inoculated with mycelia of B.berengeriana f.sp.piricola and induced under the 365 nm-UV-light;when the brown lesion was shown,the young apple began to form pycnidia on the lesion around the inoculation point after 3-5 days irradiation with UV-light and extruded plenty of conidia.This method induced great more conidia than that of irradiating wounded mycelia with UV-light.However,mature apple seldom produced conidia when induced with the same method.In order to get a great quantity of spores of Botryosphaeria berengeriana f. sp. piricola, several methods to promote sporulation of the pathogen were compared. The results showed that the wounded young apple fruits within 60-day old was inoculated with mycelia of B. berengeriana f. sp. piricola and induced under the 365 nm-UV-light; when the brown lesion was shown, the young apple began to form pycnidia on the lesion around the inoculation point after 3-5 days irradiation with UV-light and extruded plenty of conidia. This method induced great more conidia than that of irradiating wounded mycelia with UV-light. However, mature apple seldom produced conidia when induced with the same method.

关 键 词：苹果轮纹病菌 人工接种技术 产孢 诱导 病害防治 流行规律 苹果果实 病原菌 

分 类 号：S482.2[农业科学—农药学] S436.421.1[农业科学—植物保护]