小西葫芦黄花叶病毒山东南瓜分离物的分子特性  

Molecular characterization of Zucchini yellow mosaic virus isolate infecting pumpkin in Shandong

作　　者：刘金亮[1,2] 邵云华[1] 张广民[1] 竺晓平[1] 杨广玲 时呈奎[1] 李向东[1] 

机构地区：[1]山东农业大学植物保护学院,泰安271018 [2]吉林大学植物科学学院,长春130062

出　　处：《植物病理学报》2009年第5期544-548,共5页Acta Phytopathologica Sinica

基　　金：新世纪优秀人才支持计划(NCET-07-0520);山东省自然科学基金重点项目(Z2007D04);教育部博士学科点基金(200804340006)

摘　　要：Zucchini yellow mosaic virus(ZYMV) was detected by RT-PCR from pumpkin(Cucurbita moschata) plant showing yellowing and mosaic symptom from Liaocheng,Shandong Province.The 3′-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence,840 bp of cp gene and 211 bp of 3′-untranslated region of the isolate ZYMV-Liaocheng was determined.The cp gene of ZYMV-Liaocheng shared identities of 81.4%-98.8% and 89.4%-99.5% at nucleotide and amino acid levels,respectively,with other ZYMV sequences available in the GenBank.Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes.ZYMV-Liaocheng belonged to genotype I,which contained isolates from Asia,Europe and America.Genotypes III and V were unique and contained only isolates from East Asia.The isolates from East Asia had the highest variability.Zucchini yellow mosaic virus （ZYMV） was detected by RT-PCR from pumpkin （ Cucurbita moschata） plant showing yellowing and mosaic symptom from Liaocheng, Shandong Province. The 3＇-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence, 840 bp of cp gene and 211 bp of 3＇- untranslated region of the isolate ZYMV-Liaocheng was determined. The cp gene of ZYMV-Liaocheng shared identities of 81.4% - 98.8% and 89.4% - 99.5% at nucleotide and amino acid levels, respectively, with other ZYMV sequences available in the GenBank. Phylogenetic analysis indicated that ZYMV could be clustered to 6 genotypes. ZYMV-Liaocheng belonged to genotypeⅠ, which contained isolates from Asia, Europe and America. Genotypes Ⅲ and Ⅴ were unique and contained only isolates from East Asia. The isolates from East Asia had the highest variability.

关 键 词：小西葫芦黄花叶病毒 分离物 分子特性 葫芦科作物 南瓜 山东 MOSAIC ZYMV 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治] S435.663[农业科学—植物保护]