盐胡芦巴HPLC指纹图谱研究  

Fingerprints of saline semen Trigonellae by HPLC

作　　者：杨云[1] 许小华[1] 刘富岗[1] 肖功胜[1] 

机构地区：[1]河南中医学院药学院,河南郑州450008

出　　处：《中成药》2009年第11期1641-1643,共3页Chinese Traditional Patent Medicine

基　　金：河南省重大科技攻关项目(0522030200)

摘　　要：目的:建立盐胡芦巴的HPLC指纹图谱分析方法。方法:采用高效液相色谱技术,phenom enex Hydro-RP 80A(4.6mm×250 mm,5μm)色谱柱,乙腈-水为流动相进行等度与梯度洗脱,检测波长211 nm,流速1.0 mL/m in,柱温为40℃。结果:建立了以17个共有峰为特征指纹信息的HPLC指纹图谱,发现各产地采用规范化炮制工艺制备的盐胡芦巴指纹图谱无明显差异。结论:该指纹图谱方法准确可靠,重现性好,可作为盐胡芦巴内在质量评价的依据。AIM： To establish the HPLC fingerprints of semen Trigonellae processed with salt-water. METHODS：HPLC analysis was performed on a phenomenex Hydro-RP 80A（4.6 mm × 250 mm,5 μm） with mobile phase aeetonitrile-water under isocratie and gradient elutions, and detection wavelength was 211 nm, the flow rate was 1 mL/min, column temperature was 40 ℃. RESULTS： HPLC fingerprint of 17 main peaks was separated. It was found that the preparative HPLC of standardized processing saline semen Trigonellae from different places have no significant differences. CONCLUSION ： The method is reliable, accurate and can be used for quality control of saline semen Trigonellae.

关 键 词：盐胡芦巴 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]