白香丹胶囊中芍药苷、丹皮酚及α-香附酮的HPLC含量测定  

Determination of paeoniflorin,paeonol and α-cyperone in Baixiangdan Capsule by HPLC

作　　者：李英美 张彬 范晓惠 

机构地区：[1]青岛海川创新生物天然药物研究中心,山东青岛266061

出　　处：《中成药》2009年第11期1690-1694,共5页Chinese Traditional Patent Medicine

基　　金：十一五国家科技支撑计划项目(2006BAI06A18-13)

摘　　要：目的：研究白香丹胶囊（白芍提取物,丹皮酚提取物,香附挥发油）含量测定方法,并建立其质量标准。方法：采用HPLC法分别测定白香丹胶囊中芍药苷、丹皮酚、α-香附酮的含量。结果：芍药苷在0.612-5.508μg之间线性关系良好（r=0.999 3）,平均回收率为98.16%,（RSD=1.18%,n=5）;丹皮酚在0.150 4-1.353 6μg之间线性关系良好（r=0.999 8）,平均回收率为98.17%,（RSD=1.52%,n=5）;α-香附酮在0.271-2.439μg之间线性关系良好（r=0.999 9）,平均回收率为98.02%（RSD=1.58%,n=5）。结论：所用方法准确、可靠,可行性及重复性良好,可用于控制该制剂的质量。AIM： To establish the analytical methods for determing paeoniflorin,paeonol and α-cyperone in Baixiangdan Capsule（extract of Radix Paeoniae alba,extract of paeonol,volatile oil of Rhizoma Cyperi）. METHODS： The contents of paeoniflorin,paeonol and α-cyperone in the capsule were determined by HPLC respectively. RESULTS： The linear range of paeoniflorin was within 0.6125.508 μg（r=（0.999 3）） and the average recovery was 98.16%（RSD=1.18%,n=5）.The linear range of paeonol was 0.150 4 μg-1.353 6 μg（r=（0.999 8）） and the average recovery was 98.02% （ RSD = 1.58%, n = 5 ）. CONCLUSION ：The method is reliable, accurate, feasible and reproducible and can be applied to the quality control of Baixiangdan Capsule.

关 键 词：白香丹胶囊 芍药苷 丹皮酚 Α-香附酮 HPLC 

分 类 号：R927.2[医药卫生—药学]