HPLC同时测定藿香清胃颗粒中升麻素苷、5-O-甲基维斯阿米醇苷、栀子苷含量  

Determination of prim-o-glucasylcimifugin,5-o-methylvisammiosode and gardenoside in Huoxiang Qingwei Granules by HPLC

作　　者：陆静娴[1] 祝明[2] 陈勇[2] 

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江省食品药品检验所,浙江杭州310004

出　　处：《中成药》2009年第11期1701-1704,共4页Chinese Traditional Patent Medicine

摘　　要：目的：建立藿香清胃颗粒（广藿香、栀子、防风等）中升麻素苷、5-O-甲基维斯阿米醇苷和栀子苷的含量测定方法。方法：采用高效液相色谱法在同一色谱条件（色谱柱：Shimadzu VP-ODS 250 mm×4.60 mm,5μm;流动相：乙腈-0.1%磷酸梯度洗脱;流速：1.0 mL/min;柱温：30℃）下对制剂中3种成分进行定量测定。结果：升麻素苷在26.0-390 ng范围内线性关系良好,r=0.999 9,平均回收率为101.7%,RSD=0.7%;5-O-甲基维斯阿米醇苷在21.5-322.2 ng范围内线性关系良好,r=0.999 9,平均回收率为101.9%,RSD=1.1%;栀子苷在105.4-1 580.4 ng范围内线性关系良好,r=0.999 9,平均回收率为101.6%,RSD=1.1%。结论：所建立的方法可在同一色谱条件下检测出3个成分,可用于该制剂的质量控制。AIM ： To establish a method of determing prim-o-glucasylcimifugin, 5-o-methylvisammiosode, gard- enoside quantitatively in Huoxiang Gingwei Granules （Herba Pogostemonis, Fructus Gardeniae, Radix Saposhnikoviae, etc. ）. METHODS： Three components were determined by HPLC in the same chromatographic condition （ Column ： Shimadzu VP-ODS 250 mm × 4.60 mm, 5 μm ; mobile phase ： acetonitrile-0.1% phosphoric acid gradient elution ; flow rate ： 1.0 mL/min ： column temperature ： at 30 ℃ ）. RESULTS ： Prim-o-glucasylcimifugin showed a good linear relationship at the range of 26.0 -390 ng, r = 0. 999 9, the average recovery was 101.7% and RSD was 1.1%. 5-o-methylvisammiosode showed a good linear relationship at the range of 21.5 -322.2 ng, r = 0. 999 9, the average recovery was 101.9% and RSD was 1.1%. Gardenoside showed a good linear relationship at the range of 47. 1 - 706. 5 ng, r = 0.999 9, the average recovery was 101. 6% and RSD was 1. 1%.CONCLUSION： The established method can provide the content determination of 3 ingriedents at the same test condition and be used for the quality control of Huoxiang Qingwei Granules.

关 键 词：藿香清胃颗粒 HPLC 升麻素苷 5-O-甲基维斯阿米醇苷 栀子苷 

分 类 号：R927.2[医药卫生—药学]