缓冲液成分对介电泳力的影响  

Effect of Composition of Buffer to Dielectrophoresis

作　　者：许静[1,2] 赵湛[1] 刘泳宏[1,2] 方震[1] 杜利东[1,2] 耿道渠[1,2] 

机构地区：[1]中国科学院电子学研究所传感器国家重点实验室北方基地,北京100190 [2]中国科学院研究生院,北京100039

出　　处：《仪表技术与传感器》2009年第B11期78-80,共3页Instrument Technique and Sensor

基　　金：国家重点基础研究发展计划资助(973)(2009CB3200300);中国高技术研究发展计划(863)(2007AA042104)

摘　　要：在介电泳研究中,缓冲液对微粒运动情况的影响是不可忽略的,而目前关于缓冲液的研究关注则较少。文章旨在研究缓冲液中的大分子与离子对介电泳操纵的贡献。通过观察酵母菌在不同成分、不同配比的缓冲液中的运动情况,说明了缓冲液中大分子成分的增加可以降低溶液电导率而在同样电导率的溶液中对微粒的介电泳操纵影响不大,而离子的增多会增大溶液电导率,而在同样的电导率下会增加介电泳对微粒操纵的困难程度。It is significant that the buffer will affect the behavior of the particulate in the medium, but the research about this is less. Researches were done about the contribution of the big-molecules and ions to dielectrophoretic manipulate in medium. Through the behavior of the yeast cell in the medium that was different to the composition and the proportion, the experimental data prove that the increase of the big molecule reduce the conductivity of the buffer while the increase of the ion augment the conductivity of the buffer and aggravate the difficulty of dielectrophoretic manipulation to particle.

关 键 词：介电泳 缓冲液 大分子 离子 

分 类 号：TP21[自动化与计算机技术—检测技术与自动化装置]