心肌细胞传感器及其在生物医学中的应用  

Cardiac Cell-based Biosensor and Its Biomedical Applications

作　　者：刘清君[1,2] 余辉[1] 肖丽丹[1] 叶伟伟[1] 蔡华[1] 王平[1,2] 

机构地区：[1]浙江大学生物医学工程与仪器科学学院,生物传感器国家专业实验室,浙江杭州310027 [2]中国科学院,传感技术联合国家重点实验室,上海200050

出　　处：《仪表技术与传感器》2009年第B11期291-294,298,共5页Instrument Technique and Sensor

基　　金：国家自然科学基金(30700167);浙江省自然科学基金(Y2080673);传感技术联合国家重点实验室基金(Skt0702)

摘　　要：传统心肌细胞电生理的研究,因其对细胞的穿刺损害作用,难以实现长时程测量,同时也难以实现对细胞群体的多位点同时测量。该研究采用微机械加工技术开展了基于细胞传感器(cell-based biosensor)的微电极阵列(microelectrodearray,MEA)和光寻址电位传感器(light addressable potentiometric sensor,LAPS)等生物传感器技术,在传感器芯片表面培养了心肌细胞,并对其电生理特性进行了传感测量。该技术可对多个细胞同时进行长期、无损检测,在药物筛选、环境检测等生物医学领域具有良好的应用前景。The membrane recording of the cardiomyocytes electrophysiology, made it difficult to realize long-term monitoring, and the multi channels synchronous recording of cells networks, due to their sticking damage to cells. Using microeleetromeehanical systems, cell-based biosensors of microeleetrode array （MEA） and light addressable potentiometric sensor （LAPS） were developed, to record the extracellular potentials of cardiomyocytes. Cells were cultured on the sensor surface to monitor the electrical activities of cardiomyocytes in vitro. In a long-term and non-invasive way, the novel biosensor has been successfully applied primarily to biomed ical studies such as drugs screening and environment detection.

关 键 词：生物传感器 光寻址电位传感器 微电极阵列 心肌细胞 药物分析 毒性检测 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]