比克氏棉及其杂种后代的RAPD分析  

RAPD Analysis of Gossypium bickii and Its Hybrid Descendents

作　　者：渠云芳[1] 黄晋玲[1] 仪治本[2] 李炳林[1] 

机构地区：[1]山西农业大学农学院,太谷030801 [2]中北大学,太原030051

出　　处：《棉花学报》2009年第6期468-473,共6页Cotton Science

基　　金：山西省科技攻关项目(20080311001-1);山西农业大学科技创新基金(2004082)

摘　　要：将(亚洲棉×比克氏棉)F1加倍,再与陆地棉、海岛棉、(陆地棉×夏威夷棉)、(陆地棉×黄褐棉)杂交,获得三种杂种与四种杂种。本研究用RAPD技术对种间杂种及其亲本进行了分析,结果表明:(1)15个引物对参试材料总共扩增出127条DNA带,其中有102条多态性带,占80.3%;(2)在杂种中均检测到了亲本带与杂种的特异带;(3)利用SAS软件中的类平均法对试验材料进行聚类分析,结果表明,所有的参试材料聚为三大类。研究结果充分验证了杂种的真实性。The interspecific hybrids were derived from E （Gossypiurn arboreum × Gossypium bickii ） × Gossypiurn hirsuturn ] , （G. arboreum × G. bickii ） × Gossypium barbadense ] , [（G. arboreurn × G. bickii） X （G. hirsuturn X Gossypiurn mustelinurn ）], （G. arboreum X G. bickii） × （G. hirsuturn×Gossypium tornentosum）] after doubling F1 of （G. arboreurn×G, bickii）. These hybrids and their parents were analyzed by RAPD. The results showed that： （1）There were 127 amplified DNA bands from 15 primers, 102 bands （80.3 %） were polymorphic; （2） Parents bands and specific bands of hybrids were detected in hybrids; （3） The cluster analysis of materials was studied by group-average method from SAS software. Cluster analysis revealed that these materials were divided into 3 groups. All the results verified the authenticity of hybrids.

关 键 词：比克氏棉 杂种后代 RAPD 

分 类 号：S562.035.1[农业科学—作物学]