三个不同耐高温棉花基因型主茎叶的生理生化特征  

Physiological and Biochemical Characteristics of Main-stem Leaves among Three Cotton Genotypies with Varying High-temperature Tolerance

作　　者：袁小玲[1,2] 熊格生[3] 邓茳明[1] 吴锋[1] 刘志[1] 

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]湖南农大金农种业有限公司,长沙410128 [3]湖南农业大学科学技术师范学院,长沙410128

出　　处：《棉花学报》2009年第6期492-496,共5页Cotton Science

基　　金：国家自然科学基金项目(30900909);湖南省教育厅优秀青年科研项目(08B033)

摘　　要：对不同高温耐性棉花主茎叶的生理生化特性及其变化规律进行了比较研究。结果表明,耐高温棉花HLY细胞膜热稳定性显著高于敏感品系TS18,而与中度敏感品系XYM68在苗期和盛蕾期没有明显差异,到盛花和结铃盛期差异显著;三个材料之间的叶片脯氨酸含量除苗期外均存在显著差异,可溶性糖含量在结铃盛期才差异显著,而可溶性蛋白质含量差异不明显;HLY叶片叶绿素含量在盛花期和结铃盛期显著高于XYM68和TS18,而净光合速率在整个生育时期都显著高于XYM68和TS18。主茎叶细胞热稳定性、脯氨酸、可溶性糖及净光合速率能较好区分耐高温与敏感材料,这为耐高温棉花的筛选与鉴定提供了重要的生理生化指标。A comparative investigation on the physiological and biochemical characteristics and their fluctuation in main stem leaf of cotton lines with different tolerance to high temperature stress was presented in the paper. The results showed that the leaf cell membrane thermo-stability （CMT） was significantly stronger in tolerant line HLY than susceptible line TS18 during the whole growth period and moderately susceptible line XYM68 at peak flowering and boll-setting stages. Of the three differentially tolerant lines, the proline content was different significantly with each other at all stages except for seedling, the soluble sugar content became significantly different at peak flowering and bollsetting stages, while there was nearly no change for dissolvable protein content during the whole developmental period. The chlorophyll content was higher in tolerant line HLY than susceptible counterparts XYM68 and TS18 at peak flowering and boll-setting stages and HLY owned the highest net photosynthetic rate at all of the four stages. The tolerant cotton line may be distinguished from susceptible ones by the CMT, proline, soluble sugar content and net photosynthetic rate traits in main stem leaf, which can be used as a measure of high temperature tolerance in cotton breeding program.

关 键 词：棉花 高温耐性 主茎功能叶 生理生化特性 

分 类 号：S562[农业科学—作物学]