甘蓝组织培养中防止外植体褐化的研究  

Study on Preventing Browing of Explants during Brassica oleracea L. Tissue Culture

作　　者：李然红[1] 于丽杰[2] 李晓东[1] 

机构地区：[1]牡丹江师范学院生物系,黑龙江牡丹江157012 [2]哈尔滨师范大学,黑龙江哈尔滨150025

出　　处：《北方园艺》2009年第11期96-99,共4页Northern Horticulture

基　　金：黑龙江省自然科学基金资助项目(C0014);哈尔滨市学科后备带头人基金资助项目(0071007002);黑龙江省教育厅科学基金资助项目(11511122)

摘　　要：以中甘11号为试验材料,探讨基本培养基(MS、1/2MS)、PVP(聚乙烯吡咯烷酮)、Vc(抗坏血酸)、硝酸银、母株预处理对褐化防止的有效性。结果表明:基本培养基为以1/2MS时,外植体褐化减轻,与MS培养基比差异显著。抗氧化试剂抑制褐化的作用明显,PVP对褐化的抑制作用与对照比差异显著。外植体采取前对母株进行遮光处理有利于抑制褐化,且效果明显。Took Zhanggan 11 as material for the trial,discussed the affection of the basic medium（MS,1/2MS）,PVP（polyethylene pyrrole-2-one）,Vc（ascorbic acid）,AgNO3 and the pretreatment on the effectiveness of browning prevention.The results showed that on 1/2 MS medium,the browning phenomenon was lighter,with MS for the medium,the browning phenomenon was much more serious.The antioxidant played an important role in inhibiting browning.PVP inhibited the browning more effectively.Explants taken after tree pretreatment may be good for browing inhibition.

关 键 词：甘蓝 组织培养 褐化 

分 类 号：S635[农业科学—蔬菜学] S603.6[农业科学—园艺学]