紫玉兰的组织培养  

Tissue Culture of Magnolia liliflora Desr.

作　　者：陆秀君[1] 董阳[1] 金亚荣 徐石[3] 

机构地区：[1]沈阳农业大学林学院,辽宁沈阳110161 [2]凌源市林业局河东造林站,辽宁凌源122500 [3]桓仁县林业局,辽宁桓仁117200

出　　处：《北方园艺》2009年第11期189-191,共3页Northern Horticulture

摘　　要：以成年紫玉兰带芽茎段为外植体,采用不同种类的基本培养基和激素配比对紫玉兰组培技术进行了研究,培育出了紫玉兰组培苗。适宜紫玉兰增殖的培养基为:MS+NAA 0.1mg/L+BA 1 mg/L;采用二步生根法,将继代无菌苗接入MS+NAA 2 mg/L+BA 0.1 mg/L培养基暗培养20 d后,转入不加任何激素的1/2MS培养基中,15 d左右可出根。An experiment was conducted to study the tissue culture technique using shoot stems with a lateral buds of the mature tree Magnolia liliflora Desr.as explants.Tissue cultured seedlings of Magnolia liliflora Desr.were obtained by means of medium and diferent concentrations of hormone.Result showed that the optimum medium of proliferation was MS＋NAA 0.1 mg/L＋BA 1 mg/L.Two steps uesed in rooting,the buds were planted into the medium：MS＋NAA 2 mg/L＋BA 0.1 mg/L, after 20 days under darkness, the buds were transferred into the medium： 1/2 MS without any hormone,rooting started at about 15 days.

关 键 词：紫玉兰 组织培养 培养基 

分 类 号：S685.15[农业科学—观赏园艺]