哺乳期不同营养状态对雌鼠性发育及下丘脑促性腺激素释放激素神经元表达的影响  

Effects of Different Nutrition Suckling on Hypothalamus Gonadotropin-Releasing Hormone Neurons Expression and Adolescence Sexual Development in Suckling Female Rats

作　　者：崔璐璐[1] 李嫔[1] 许丽雅[1] 朱志颖[1] 

机构地区：[1]上海交通大学附属儿童医院内分泌科,上海200040

出　　处：《实用儿科临床杂志》2009年第20期1557-1559,共3页Journal of Applied Clinical Pediatrics

基　　金：上海科委自然科学基金项目资助(06ZR14053)

摘　　要：目的通过建立哺乳期不同营养状态雌鼠模型,观察不同哺乳期营养状态对雌鼠阴门开启时间及下丘脑弓状核促性腺激素释放激素(GnRH)神经元表达,探讨哺乳期营养状态对雌鼠青春期性启动及性发育的影响。方法将1日龄SD雌鼠80只随机分为3组:营养过剩组(2只仔鼠由1只母鼠哺育),营养适中组(10只仔鼠由1只母鼠哺育),营养不良组(20只仔鼠由1只母鼠哺育)。仔鼠21日龄断奶,饲以普通实验室饲料。观察阴门开启时间,分别于营养过剩组及营养适中组所有雌鼠阴门开启时,处死3组雌鼠,每组10只。利用免疫组织化学方法检测各组雌鼠下丘脑弓状核GnRH阳性神经元数、阳性神经元百分率。结果1.当营养过剩组所有雌鼠阴门开启时,即35日龄时,雌鼠GnRH阳性神经元数,营养过剩组为(247±111)个,营养适中组为(143±73)个,营养不良组为(74±70)个,营养过剩组明显高于营养适中组及营养不良组(Pa<0.05)。2.当营养适中组所有雌鼠阴门开启时,即42日龄时,雌鼠GnRH阳性神经元数,营养过剩组为(266±98)个,营养适中组为(206±68)个,营养不良组为(106±83)个,营养不良组明显低于营养过剩组及营养适中组(Pa<0.05);但营养过剩组与营养适中组比较差异无统计学意义(P>0.05)。3.各组雌鼠阴门开启时间:营养过剩组为(29.60±1.96)d;营养适中组为(39.10±1.66)d;营养不良组为(41.30±3.33)d,组间比较差异具有统计学意义(Pa<0.05)。结论哺乳期营养状态对雌鼠性发育有显著影响,营养过剩雌鼠性发育启动早,GnRH神经元较早出现分泌高峰,营养不良雌鼠性发育启动晚,GnRH神经元分泌GnRH激素延迟。Objective To explore the adjustment effects of suckling nutrition on hypothalamus gonadotropin - releasing hormone neurons （GnRH） expression level and adolescence sexual development by establishing different nutrition models for suckling female rats. Methods One - clay - old female Sprague - Dawley rats were randomly assigned to 3 groups. Two suckling rats were fed by one laetation rat in overnutriion group, 10 suckling rats were fed by one lactation rat in normal control group ,20 suckling rats were fed by one lactation rat in malnutrition group. The animals were weaned at day 21 of age in groups of 5 rats per cage with free access to pelleted food and tap water. To comparatively observe vaginal opening, 10 rats in each group were randomly sacrificed when the overnntrition rats showed complete vaginal opening and when the normal control rats showed complete vaginal opening. The amount and percentage of the positive GnRH neurons in hypothalamus areuate nucleus were detected with immunohistoehemistry. The influence of suckling nutrition to female rats adolescence sexual development was assessed by statistical analysis. Results 1. When the overnutrition rats showed complete vaginal opening （ at 35 - day - old）, the amount of positive GnRH neuron in hypothalamus arcuate nucleus was respectively 247 ± 111 in overnntrition group, 143 ± 73 in normal control group and 74 ± 70 in malnutrition group. It was showed that the amount of the positive GnRH neuron of hypothalamus arcuate nucleus in overnutrition group was more than the other groups（ Pa 〈 0.05 ）. 2. When the normal control rats showed complete vaginal opening（ at 42 -day -old）, the amount of positive GnRH neuron in hypothalamus arcuate nucleus was respectively 266 ± 98 in overnutrition group ,206 ±68 in normal control group and 106 ± 83 in malnutrition group. It demonstrated that the amount of positive GnRH neuron of hypothalamus in malnutrition group was less than that in normal control group and ovemutrition group（ P 〈 0.05 ）. Neve

关 键 词：促性腺激素释放激素 哺乳期 营养 性发育 大鼠 

分 类 号：R723[医药卫生—儿科]