血吸虫病肝纤维化实验动物模型的建立与评价  

Evaluation of the Animal Model of Hepatic Fibrosis Caused by Schistosomiasis

作　　者：何永康[1,2] 周杰[2] 喻鑫玲[2] 刘宇[1] 聂东宋[1] 候循亚[2] 孙渊[2] 朱剑君[2] 赵雅琴[2] 李岳生[1,2] 

机构地区：[1]湖南理工学院,岳阳414000 [2]湖南省血吸虫病防治所,岳阳414000

出　　处：《热带医学杂志》2009年第10期1147-1148,1160,F0004,共4页Journal of Tropical Medicine

基　　金：湖南省科技厅专项(湘财教指2008.115)

摘　　要：目的建立及评估血吸虫病肝纤维化实验动物模型。方法以34只日本大耳白兔为实验动物,随机分为3组,即高剂量组(A组)、低剂量组(B组)及对照组(C组)建立肝纤维化模型,每只感染日本血吸虫尾蚴100条,感染后12～13周,高剂量组和低剂量组采用清肝口服液(DHT)进行干预治疗;感染后18周,按吡喹酮剂量300mg/kg体重对3组动物进行杀虫治疗,以病理学和血清HYP、HA水平作为模型评估指标。结果感染后第12～13周,肝纤维化模型形成,从病理学结果、生化指标均产生了不同程度的改变。A组、B组干预治疗后血清中的HYP/HA较干预前明显降低(P<0.01);肝组织病理学结果显示:肝纤维化程度依次为高剂量组<低剂量组<对照组(P<0.01),且血清HYP、HA含量随着病理肝纤维化程度增高而升高。结论检测血清HYP和HA水平是评价和考核肝纤维化治疗效果的简便、快速、可靠、又无损伤的指标,而且与病理学检查结果相符,具有实际应用价值。Objective To establish and evaluate animal model of hepatic fibrosis caused by schistosomiaisis. Methods 34 rabbits, each was challenged by 100 S.j cercariae, were induced to hepatic fibrosis. 12-13 weeks post challenge, liver-clearing oral medicine （DHT） and praziquantel （PQT） were adopted as intervene therapies. Those rabbits with hepatic fibrosis were distributed into 3 groups randomly： high dose group （group A）; low dose group （group B） and control group （group C）. The pathology of hepatic tissue and HYP ＆ HA levels in serum were served as index of model evaluation. Results 12-13 weeks post challenge,the animal model of hepatic fibrosis was established. The pathology and HYP/HA level in serum were changed in different degrees. The HYP/HA levels in serum of A, B groups pre and post therapy intervention showed a significant decrease （P〈0.01）. And the results of pathology of liver tissues and HYP/HA levels showed that the degrees of hepatic fibrosis and serum levels of HYP/HA in high dose group〈low dose group〈control group （P〈0.01）. Conclusion The detection of the changes of HYP/ HA levels in serum is a convenient, rapid, reliable and noninvasive index in evaluating the therapeutic effect on hepatic fibrosis and also the changes of HYP/HA level in serum are correspond with the results of pathology,so the detection of those indices has a pragmatic value in application.

关 键 词：实验动物 模型 日本血吸虫 肝纤维化 评价 

分 类 号：R532.21[医药卫生—内科学]