硒化壳聚糖对体外培养表皮细胞增殖的影响  

Effect of selenium chitosan on the proliferation of the in vitro cultured epidermal cell

作　　者：邓守恒[1] 杨敬宁[2] 曹凤军[1] 蔡晓军[1] 邓守平[3] 陈萍[1] 

机构地区：[1]郧阳医学院附属人民医院肿瘤中心,十堰442000 [2]郧阳医学院免疫教研室 [3]郧阳医学院附属人民医院消化内科

出　　处：《山西医科大学学报》2009年第11期982-985,共4页Journal of Shanxi Medical University

基　　金：郧阳医学院附属太和医院基金资助项目(20031207)

摘　　要：目的研究硒化壳聚糖对体外培养的表皮细胞增殖的影响。方法用不同剂量(25,50,100,200,400mg/L)硒化壳聚糖作用于表皮细胞,镜下观察药物对细胞形态的影响;MTT法、3H-TdR掺入法、细胞生长动力学研究用于检测药物对细胞增殖影响,并以等体积的细胞培养液处理为对照组。结果各药物浓度组处理细胞后,细胞形态未见明显改变,均可使细胞吸光度值增加(除25mg/L组外,均P<0.05),细胞倍增时间缩短(除25mg/L组外,均P<0.05),促进表皮细胞对3H-TdR的掺入(P<0.05,P<0.01)。结论硒化壳聚糖对体外培养表皮细胞增殖具有促进作用。Objective To explore the effects of selenium chitosan on the proliferation of in vitro cultured epidermal cells. Methods The cultured epidermal ceils were treated with selenium chitosan at different concentrations of 25,50, 100,200,400 mg/L, and treated with the equal volume of media as controls. The change of epidermal cell morphology was observed under light microscope. The effect on cell proliferation was measured by MTT analysis,3 H-TdR infiltration and cell growth curve analysis. Results Compared with control group,there was no significant difference in the morphology of epidermal cells in 25,50,100,200,400 mg/L group. After treated with different concentrations of selenium chitosan, the absorbance at 540 nm increased, and the time of cell population doubling was shortened. The ^3 H-TdR infiltration levels significantly increased in 25,50,100,200,400 mg/L group compared with control group （P 〈 0.05 or P 〈 0.01 ）. Conclusion Selenium chitosan could promote the proliferation of epidermal cells cultured in vitro.

关 键 词：硒化壳聚糖 表皮细胞 增殖 

分 类 号：R318[医药卫生—生物医学工程]