不同分化潜能的真皮干细胞膜蛋白初步筛选研究  

Preliminary Study of Membrane Protein of Dermal Stem Cell with Different Potential

作　　者：陈付国[1] 毕丹[1] 张小平[1] 刘阳[1] 程开祥[1] 张文杰[1] 刘伟[1] 崔磊[1] 曹谊林[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院整复外科,上海市组织工程研究重点实验室,上海市200011

出　　处：《组织工程与重建外科杂志》2009年第5期245-249,共5页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：国家自然科学基金(30800231);上海市自然科学基金(07ZR14068)

摘　　要：目的寻找不同分化潜能的真皮干细胞的差异膜蛋白。方法通过选择性地抽提三向分化克隆的细胞膜蛋白,与二向分化克隆进行对比分析,以筛选出与分化能力下降有关的细胞膜相关蛋白。三向克隆与双向克隆的膜蛋白经2-D电泳、质谱分析及Western-Blot检测,初步确认二者之间的明显差异蛋白。结果蛋白质组学分析结果显示,二者的差异蛋白主要有10种膜相关蛋白,其中膜表达差异较显著的蛋白质,如:Annexin A2 isoform1(ANXA2)、Prohibitin(PHB)、Protein disulfide-isomeraseA3(PDIA3),均与细胞分化有关。结论ANXA2、PHB、PDIA3在不同分化潜能的人真皮干细胞膜上表达存在差异,提示了这几组蛋白质参与了该细胞的分化,为初步筛选多潜能真皮成纤维细胞,以及进一步研究其分化的机理提供了重要科学依据和深入探索的新方向。Objective The membrane protein of clonal population with different potential （tri-clone VS bi-elone） was analyzed in this study. Methods Protein extracts were prepared from cultured elonal population of human dermis and were analyzed by 2D-DIGE. Differentially expressed proteins were identified by mass spectrometry （MS）. Results Significant different expression was observed for 22 proteins with 10 under-expressed membrane proteins. Among them, annexin A2 isoform 1 （ANXA2）, prohibitin （PHB） and protein disulfide-isomerase A3 （PDIA3）had significant difference of protein expression. Conclusion The expression of ANXA2, PHB and PDIA3 on the membrane of muhipotent adult cells from the dermis were different. It provided important scientific basis for screening multipotent derm fibroblasts.

关 键 词：成纤维细胞 真皮干细胞 克隆 膜蛋白 

分 类 号：Q813.11[生物学—生物工程]