小干扰RNA分子对增生性瘢痕成纤维细胞增殖的影响  

Effects of Specific Small Interfering RNA on Cell Proliferation of Human Hypertrophic Scar Fibroblasts

作　　者：陈蕊[1] 钱柳[1] 张冬青[1] 王琳[1] 傅敏刚[1] 陆毅[1] 范志宏[1] 

机构地区：[1]上海交通大学医学院附属仁济医院整形外科,上海市200127

出　　处：《组织工程与重建外科杂志》2009年第5期276-279,共4页Journal of Tissue Engineering and Reconstructive Surgery

基　　金：上海市教育委员会科研创新项目(08YZ40);仁济医院基础合作基金(PY07010)

摘　　要：目的使用RNA干扰(RNA interference,RNAi)技术抑制黏着斑激酶(Focal adhesion kinase,FAK)基因的表达,观察增生性瘢痕成纤维细胞在生物学行为上发生的变化。方法设计特异性探针合成FAK SiRNA片段,脂质体法转染体外培养的增生性瘢痕成纤维细胞,光学显微镜观察细胞形态;MTT检测细胞活性并绘制细胞生长曲线;流式细胞仪检测细胞周期。结果转染后的成纤维细胞呈短梭形改变,胞质、突触减少,细胞核内核仁减少。细胞生长曲线表明,转染FAK siRNA的瘢痕成纤维细胞生长明显抑制。转染后瘢痕成纤维细胞阻滞在G1期,S期和G2期细胞比例减少。结论FAK与瘢痕增生相关密切,因此改变FAK在成纤维细胞中的表达可望调控增生性瘢痕成纤维细胞的生物活性。Objective To investigate the effect on a diverse array of cellular processes of human hypertrophic scar fibroblasts by using FAK specific small interfering RNA （SiRNA） on inhibiting FAK gene expression. Methods FAK siRNA was design with specific target finder and transfected into hypertrophic scar fibroblasts by the liposome-mediated gene transfection method. Light microscope was used to observe cell morphologic change. The viable cells were counted by MTF colorimetry and a cell growth curve was drawn. Flow cytometry was used to examine cell cycle. Results Abnormal change of cell morphology became into short fusiform, less cytoplasm synapses and less nucleoli in cell nucleolus after transfection. The cell growth curves indicated the prolifertation of cell trasfected with FAK specific SiRNA was inhibited significantly compared with controls. Transfected hypertrophic scar fibro blasts were blocked in G1 stage. The proportion of the cells decreased in S and G2 stage. Conclusion The quantity of FAK expression may has a significant inverse correlation with the forming of hypertrophic scar, The cellular process of hypertrophic scar fibroblasts can be controlled by regulating the expression of FAK.

关 键 词：增生性瘢痕 黏着斑激酶 小干扰核糖核酸 细胞周期 成纤维细胞 

分 类 号：Q786[生物学—分子生物学]