Genetic analysis and molecular mapping of a novel gene for zebra mutation in rice (Oryza sativa L.)  

作　　者：Qiushi Wang,Xianchun Sang,Yinghua Ling,Fangming Zhao,Zhenglin Yang,Yunfeng Li,Guanghua He Key Laboratory of Biotechnology and Crop Quality Improvement,Ministry of Agriculture Rice Research Institute,Southwest University,Chongqing 400716,China 

出　　处：《Journal of Genetics and Genomics》2009年第11期679-684,共6页遗传学报（英文版）

基　　金：funded by the Excellent Youth Foundation Project of Chongqing (No. 2008BA1033);the Fine Animals and Plants Breeding Project of Chongqing (No. 2007AA1019, 2007AA1012 and 2007AB1033);the National Key Technology R&D Program of China (No. 2006BAD01A01)

摘　　要：A novel zebra mutant, zebra-15, derived from the restorer line JinhuilO （Oryza sativa L. ssp. indica） treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased （55.4%） and the ratio of Chla/Chlb increased （90.2%） significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F2 segregation populations derived from the cross of XinonglA and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the XinonglA and the zebra-15, their F2 population was then used for gene mapping. Zebra-15 （Z-15） gene was primarily restricted on the short arm of chromosome 5 by 150 F2 recessive individuals, 19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location, and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F2 recessive individuals.A novel zebra mutant, zebra-15, derived from the restorer line JinhuilO （Oryza sativa L. ssp. indica） treated by EMS, displayed a distinctive zebra leaf from seedling stage to jointing stage. Its chlorophyll content decreased （55.4%） and the ratio of Chla/Chlb increased （90.2%） significantly in the yellow part of the zebra-15, compared with the wild type. Net photosynthetic rate and fluorescence kinetic parameters showed that the decrease of chlorophyll content significantly influenced the photosynthetic efficiency of the mutant. Genetic analysis of F2 segregation populations derived from the cross of XinonglA and zebra-15 indicated that the zebra leaf trait is controlled by a single recessive nuclear gene. Ninety-eight out of four hundred and eighty pairs of SSR markers showed the diversity between the XinonglA and the zebra-15, their F2 population was then used for gene mapping. Zebra-15 （Z-15） gene was primarily restricted on the short arm of chromosome 5 by 150 F2 recessive individuals, 19.6 cM from marker RM3322 and 6.0 cM from marker RM6082. Thirty-six SSR markers were newly designed in the restricted location, and the Z-15 was finally located between markers nSSR516 and nSSR502 with the physical region 258 kb by using 1,054 F2 recessive individuals.

关 键 词：molecular mapping rice （Oryza sativa L.） zebra mutant CHLOROPHYLL Zebra-15 

分 类 号：S511[农业科学—作物学] Q78[生物学—分子生物学]