中国肥胖患者β_2肾上腺素受体基因多态性的研究  

Polymorphism in β_2 Adrenergic Receptor Gene in Chinese Population with Obesity

作　　者：吴红梅[1] 白怀[1] 范平[1] 刘瑞[2] 刘宇[3] 刘秉文[3] 

机构地区：[1]四川大学华西第二医院遗传代谢性疾病及围生医学实验室,成都610041 [2]四川大学华西医院人类疾病相关多肽研究室 [3]四川大学华西基础医学与法医学院生物化学与分子生物学教研室

出　　处：《四川大学学报（医学版）》2009年第6期1056-1061,共6页Journal of Sichuan University(Medical Sciences)

基　　金：国家自然科学基金(批准号39770322);四川大学华西第二医院科研启动基金资助

摘　　要：目的研究β2肾上腺素受体(β2 adrenergic receptor,β2 AR)基因多态性是否与中国人肥胖有关联,为探讨肥胖的分子遗传基础提供依据。方法应用多聚酶链反应-限制性片段长度多态性分析法(PCR-RFLP),对成都地区396名汉族人(270例非肥胖者及126例肥胖者)β2 AR基因Arg16Gly和Gln27Glu多态性位点进行分析。结果β2AR基因Arg16Gly位点Arg、Gly等位基因的频率在肥胖组为0.571、0.429,在非肥胖组为0.559、0.441;Gln27Glu位点Gln、Glu等位基因的频率在肥胖组为0.920、0.080,在非肥胖组为0.916、0.084。上述位点在两组间等位基因的频率差异均无统计学意义(P>0.05)。在Arg16Gly位点,非肥胖组女性和肥胖组男性Arg/Arg基因型携带者血清TC和LDLC水平分别高于Arg/Gly和Gly/Gly基因型携带者(P<0.05);此外,apoB100水平在女性非肥胖组Arg/Arg型者及肥胖组Arg/Gly型者分别升高和降低,apoAⅠ、apoAⅡ水平分别在肥胖和非肥胖男性Arg/Arg型者降低。Gln27Glu多态位点各基因型亚组间血脂和载脂蛋白水平在非肥胖组和肥胖组差异均无统计学意义(P>0.05)。结论β2AR基因Arg16Gly和Gln27Glu多态性与成都地区中国汉族人肥胖无关联,但Arg16Gly位点对特定性别非肥胖及肥胖人群血清TC、LDLC及部分载脂蛋白水平有一定影响。Objective To investigate the variation of β 2 adrenergic receptor（β 2AR） gene and its association with obesity in Chinese population.Methods The allele of β 2AR gene at Arg16Gly and Gln27Glu sites were analysed with polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） in 396 people with Han nationality in Chengdu,among whom 126 had obesity.Results The allele frequencies of Arg and Gly at Arg16Gly site were 0.571 and 0.429 for the obese people,and 0.559 and 0.441 for the non-obese people,respectively.The allele frequencies of Gln and Glu at Gln27Glu site were 0.920 and 0.080 for the obese people,and 0.916 and 0.084 for the non-obese people,respectively.No significant differences were found in the genotype frequencies of the two sites between non-obese and obese people.The non-obese females and obese males with genotype Arg/Arg at Arg16Gly site had elevated serum TC and LDL-C levels compared with those who carried Arg/Gly or Gly/Gly（P〈0.05）.The apoB100 levels increased in the non-obese females with genotype Arg/Arg and decreased in the obese females with Arg/Gly（P〈0.05）.The apoAⅠand apoAⅡlevels decreased in the obese and non-obese males with Arg/Arg（P〈0.05）.Conclusion The polymorphism in β 2AR gene at Arg16Gly and Gln27Glu is not associated with obesity in Chinese Han population.But they may have certain degree of gender-specific impact on serum TC,LDL-C and apolipoprotein levels.

关 键 词：肥胖 Β2肾上腺素受体 基因多态性 

分 类 号：R589.2[医药卫生—内分泌]