莪术对大鼠结肠平滑肌收缩的促进作用及机制  

Rhizoma curcumae promotes the contraction of rat colonic smooth muscle strips in vitro

作　　者：吕涛[1] 魏睦新[1] 

机构地区：[1]南京医科大学第一附属医院中医科,江苏省南京市210029

出　　处：《世界华人消化杂志》2009年第26期2718-2721,共4页World Chinese Journal of Digestology

基　　金：江苏省兴卫工程中医药领军人才基金资助项目;No.苏卫规财2007/158;江苏省六大人才高峰专项基金资助项目;No.07-B-015(IB07)~~

摘　　要：目的:观察莪术对离体大鼠结肠平滑肌收缩运动的影响,并初步探讨其作用机制.方法:制备大鼠结肠平滑肌肌条,以9g/LNaCl溶液(NS)为对照组,观察不同浓度莪术对结肠平滑肌的收缩效应;以莪术为对照组,分别观察酚妥拉明、维拉帕米和阿托品3种阻断剂孵育肌条后,莪术对肌条的收缩效应.结果:莪术高浓度组对离体大鼠结肠平滑肌的促进作用明显,与NS对照组比较,莪术1g/L、10g/L组引起的肌条最大收缩振幅和曲线下面积均有统计学意义(63.92±2.06,76.27±2.28vs100%;44.09±11.10,55.66±10.29vs100%,P<0.05或<0.01);与单独莪术组比较,阻断剂维拉帕米和阿托品对莪术引起的肌条收缩有抑制作用,其最大收缩振幅和曲线下面积均有统计学意义(87.35%±50.49%,73.80%±9.37%vs100%,33.97%±15.18%,27.55%±11.56%vs100%,P<0.05或<0.01).结论:莪术对大鼠结肠平滑肌的收缩活动有兴奋作用,且与剂量呈正相关,其引起的收缩效应可被阿托品和维拉帕米阻断,而未能被酚妥拉明阻断.AIM： To determine the effect of Rhizoma curcumae （RC） on the contraction of rat colonic smooth muscle strips in vitro and investigate the mechanisms involved. METHODS： Rat colonic longitudinal smooth muscle strips were prepared and treated with sodium chloride solution （9 g/L, control group） or different concentrations of RC （treatment group） to observe the effects of RC on the spontaneous contraction of the strips. Besides, the impact of verapamil, phentolamine and atropine on RC-induced contraction of colonic smooth muscle strips was examined by incubating each of these drugs with the strips. RESULTS： RC of different concentrations （0.01-10 g/L） promoted the spontaneous contraction of colonic muscle strips in vitro in a dose-dependent manner. The peak contraction amplitude and the area under the curve of contraction of colonic smooth muscle strips were significantly lower in the treatment group （RC at a concentration of 1 or 10 g/L） than in the control group （63.92% ± 2.06% and 76.27% ± 2.28% vs 100%, and 44.09% ± 11.10% and 55.66% ± 10.29% vs 100%, respectively; P 〈 0.05 or 0.01）. Atropine and verapamil could significantly decrease the peak contraction amplitude and the area under the curve of contraction of colonic smooth muscle strips treated with RC （87.35% ± 50.49% and 73.80% ± 9.37% vs 100%, and 33.97% ± 15.18% and 27.55% ± 11.56% vs 100%, respectively; all P 〈 0.05 or 〈 0.01）. CONCLUSION： RC promotes the spontaneous contraction of rat colonic smooth muscle strips in vitro perhaps via an calcium- and M-cholinergic receptor-dependent but α-adrenoceptor receptor-independent mechanism.

关 键 词：中药 莪术 结肠 平滑肌 促进 

分 类 号：R285.5[医药卫生—中药学]