温热对HPV感染皮肤朗格汉斯细胞磷酸肌醇3-激酶活性的影响  

Effects of local hyperthermia on phosphatidylinositol 3-kinase gene expression in Langerhans cells from human papillomavirus （HPV）-infected skin

作　　者：李晓东[1] 高兴华[1] 洪玉晓[1] 祝立丽[1] 王雅坤[1] 刘宇博[1] 霍玮[1] 王晓琴[1] 张丽[1] 陈洪铎[1] 

机构地区：[1]中国医科大学附属第一医院皮肤科、卫生部免疫皮肤病学重点实验室,沈阳110001

出　　处：《中华皮肤科杂志》2009年第11期756-759,共4页Chinese Journal of Dermatology

基　　金：国家自然科学摹金（30740082）;教育部创新团队发展项目（IRT0760）;沈阳市科学技术计划项目（1081267-9-00）;教育部博士点专项基金（20070159020）

摘　　要：目的探讨不同局部温热条件对HPV感染皮损游走朗格汉斯细胞（LC）磷酸肌醇3激酶（P13一K）活性的影响。方法利用37℃，42℃，45℃的温热处理培养液中的离体正常皮肤组织和尖锐湿疣皮损，采用荧光实时定量PCR法检测游走至培养液中纯化的CD1a^＋LCPI3～Kp85α、p110αmRNA的表达水平。结果正常人皮肤组织和尖锐湿疣组织游走纯化的CD1a＋LC中PI3-Kp85α、p110α mRNA的表达量均随局部温度升高而逐渐减少。正常皮肤组织游走纯化的CD1a＋LC中PI3-Kp85α mRNA的相对表达量37℃为1．00±20．00，42℃为0．78±0．13，45℃为0．54±0．17；PI3-Kp110α mRNA在不同温度的相对表达量分别为1．00±0．00，0．80±0．11，0．61±0．12；不同温度间比较，差异均有统计学意义（P〈0．01）。而尖锐湿疣组织游走纯化的CD1α＋LC中PI3-K p85α mRNA的相对表达量37℃为1．00±0．00，42℃为0．20±0．11，45℃为0．1±0．08；PI3-K p110α mRNA的相对表达量分别为1．00±0．00，0．49±0．21，0．09±0．03；不同温度间比较，差异均有统计学意义（P〈O．01）。温热对尖锐湿疣组织游走LC中PI3-K活性影响显著高于正常皮肤组织。结论局部温热可能通过抑制LC PI3-K的活性，提高机体免疫应答反应能力，消除HPV感染。Objective To investigate the effect of local hyperthermia on the mRNA expression of phosphatidylinositol 3-kinase （PI3-K） in Langerhans cells （LCs） transmigrating from HPV-infected skin. Methods Tissue samples were collected from 5 female patients with condyloma acuminatum （CA） and 5 normal human controls, then equally divided into three parts to receive local hyperthermia treatment at 37 ℃, 42 ℃ and 45 ℃, respectively, for 30 minutes. After another 12-hour incubation in RPMI 1640 culture medium, transmigrating cells were collected and LCs were purified. Real-time quantitative reverse transcription-PCR was performed to detect the mRNA expressions of PI3-K p85a and p110s in purified CDla＋ LCs from these tissue samples. Results In CDIa＋ LCs transmigrating from normal skin and lesions of CA, the expressions of PI3-K p85a and p110α mRNA decreased with the increase of temperature. After local hyperthermia treatment of normal skin at 37 ℃, 42 ℃, 45 ℃, the relative mRNA expression level was 1.00 ± 0.00, 0.78 ± 0.13, 0.54 ± 0.17, respectively, for PI3-K p85α in transmigrating LCs, 1.00 ± 0.00, 0.80 ± 0.11, 0.61 ± 0.12, respectively, for PI3-K p110α; there was a significant difference among the three temperatures in both parameters （both P 〈 0.01 ）. ha the case of lesions of CA, the relative mRNA expression level of PI3-K p85α and p110α was 1.00 ± 0.00 and 1.00 ± 0.00 under treatment at 37 ℃, 0.20 ± 0.11 and 0.49 ± 0.21 at 42 ℃, 0.1 ± 0.08 and 0.09 ± 0.03 at 45 ℃, respectively; significant difference was also noted among the three treatment temperatures （both P 〈 0.01 ）. The decrease in PI3-K p110α mRNA expressions under hyperthermia treatment at 42 ℃ and 45 ℃ compared with those at 37 ℃ was greater in LCs from CA lesions than that from normal skin. Conclusions Local hyperthermia could remarkably inhibit the expression of PI3-K genes, which may enhance immunity and favor elimination of HPV.

关 键 词：郎格尔汉斯细胞 1-磷脂酰肌醇3-激酶 高温 诱发 

分 类 号：R752.53[医药卫生—皮肤病学与性病学]