卡泊三醇对黑素细胞黑素合成的影响  

Effect of calcipotriol on melanin synthesis by human melanocytes

作　　者：汤芦艳[1] 傅雯雯[1] 张勇[1] 祝绿川[1] 郑志忠[1] 

机构地区：[1]复旦大学附属华山医院皮肤科,上海200040

出　　处：《中华皮肤科杂志》2009年第11期771-773,共3页Chinese Journal of Dermatology

摘　　要：目的探讨卡泊三醇对黑素细胞黑素合成的影响及其作用的可能机制。方法用噻唑蓝比色法（MTr法）、酶学方法及NaOH法，观察卡泊三醇对黑素细胞增殖及色素合成的作用；RT—PCR和蛋白印迹分别检测卡泊三醇对黑素细胞酪氨酸酶基因转录和蛋白表达水平的影响。结果10^-9～10^-5mol／L浓度范围的卡泊三醇对体外培养的黑素细胞增殖的影响与空白对照组比较差异无统计意义（P〉0．05）。同样浓度范围下，10^-9、10^-8mol／L浓度的卡泊三醇能明显增强黑素细胞的酪氨酸酶活性和促进黑素细胞的黑素含量，与空白对照组比较，酪氨酸酶活性可分别升高137％、123％（P〈0．05），黑素含量分别增加40．63％、18．75％（P〈0．05）。其中10^-9mol／L浓度的卡泊三醇增强黑素细胞的酪氨酸酶活性及促进黑素细胞的黑素含量最明显。与高浓度组、空白对照组比较，10^-9mol／L浓度的卡泊三醇使酪氨酸酶蛋白表达增高也最明显，较空白对照组增高270．4％（P〈0．05）。结论卡泊三醇对黑素细胞增殖无影响，可增加黑素细胞酪氨酸酶蛋白表达水平，提高酪氨酸酶活性，从而促进黑素细胞的黑素合成。Objective To investigate the effect of calcipotriol on melanin synthesis by human melanocytes and its possible action mechanism. Methods Primary melanocytes were cultured with various concentrations （10^-5, 10^-6, 10^-7, 10^-8, 10^-9, 10^-10 mol/L） of calcipotriol for 24 or 48 hours. Subsequently, MTT assay, NaOH assay, Dopa-oxidase assay, Western blot and semiquantitative RT-PCR were used to measure the cell proliferation of, melanin synthesis by, tyrosinase activity, protein and mRNA expression levels in the melanocytes, respectively. Those untreated melanocytes served as the control. Results The calcipotriol between 10^-9 and 10-5 mol/L had no significant effect on the proliferation of cultured melanocytes （P 〉 0.05）, while that of 10^-9 and 10^-8 mol/L increased tyrosinase activity by 137% and 123%, and enhanced melanin synthesis by 40.63% and 18.75%, respectively, compared with untreated melanocytes （both P 〈 0.05）. Moreover, the tyrosinase protein level increased by 270.4% （P 〈 0.05） in melanocytes treated with calcipotriol at 10^-9 mol/L for 24 hours. The strongest tyrosinase activity and melanin synthesis was observed in melanocytes treated with calcipotriol of 10 ^-9 mol/L. Conclusions The proliferation of melanocytes is unaffected by calcipotriol at 10^-9 to 10^-5 mol/L, but it can elevate the expression of tyrosinase protein, enhance tvrosinase activity, and promote melanin synthesis in melanocvtes.

关 键 词：黑素细胞 黑素类 骨化三醇 一元酚单氧酶 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]