利用微型生物光学传感器的竞争免疫检测法检测牛奶中氨苄青霉素(英文)  

Development of a Miniature Optical Biosensor-Based Inhibition Immunoassay for Detection of Ampicillin Residues in Milk

作　　者：张增福[1] 刘瑾[1] 徐可欣[1] 石婷[1] 

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室,天津300072

出　　处：《纳米技术与精密工程》2009年第6期483-489,共7页Nanotechnology and Precision Engineering

基　　金：国家自然科学基金资助项目(30700146,30800239);教育部博士点基金资助项目(20070056075);天津市应用基础与前沿技术研究计划资助项目(06YFGDGX17400)

摘　　要：利用微型表面等离子体共振的生物传感器测量了残留在牛奶中的氨苄青霉素的浓度.采用竞争抑制试验的方法,即先将定量的单克隆抗氨苄青霉素抗体(3H295)和含氨苄青霉素的牛奶样品混合,样品中氨苄青霉素即与抗体结合,然后将该混合样品通入传感器表面,传感器的表面共价固定了氨苄青霉素分子,通过生物特异相互作用分析,检测样品中剩余的抗体,从而得到氨苄青霉素的浓度.样品的测量时间为10 m in,最低检测限为2.5 ng/mL,低于欧盟标准4 ng/mL.该检测方法的测量时间短、重复性好,批间测量的变异系数为5.4%,表明该方法能满足实际测量要求.The development of an immunoassay for the detection of ampicillin residues in milk using a miniature surface plasmon resonance （SPR） biosensor was reported in this paper. The assay was based on a competitive inhibition assay with ampicillin covalently immobilized on the sensor surface. Milk samples with ampicillin residues were first incubated with a fixed amount of anti-ampicillin monoclonal anti-body （AMA） 3H295 ,whereby ampicillin in the positive samples bound to AMA. Non-complexed AMA in the sample was then allowed to form a complex with the ampicillin immobilized on the sensor chip. Then the amount of free AMA in the sample was detected in an SPR-based biospecific interaction analysis （BIA）. The immunoassay results are available in 10 min and the limit of detection is 2.5 ng/mL , lower than the European maximum residue limit （MRL） of 4 ng/mL. With short assay time and good repeatability between runs （ or coefficient of variation of 5.4% at 2.5 ng/mL）, this assay method is suitable for routine use.

关 键 词：表面等离子体共振 生物传感器 免疫检测 氨苄青霉素 牛奶 

分 类 号：TP29-A8[自动化与计算机技术—检测技术与自动化装置]