神经肽Y对大鼠心肌细胞肌浆网钙的影响  

EFFECT OF NEUROPEPTIDE Y ON CALCIUM IN SARCOPLASMIC RETICULUM OF RAT CARDIOMYOCYTES

作　　者：张弋[1] 苏涛[1] 刘世明[1] 董颀[2] 陈敏生[1] 

机构地区：[1]广州医学院第二附属医院,广东广州510260 [2]广州医学院生理教研室,广东广州510182

出　　处：《现代医院》2009年第11期9-11,共3页Modern Hospitals

基　　金：国家自然科学基金资助项目(编号:30440053;30570759);广东省自然科学基金资助项目(编号:04004529)

摘　　要：目的观察神经肽Y(NPY)刺激对大鼠心肌细胞肌浆网(Sarcoplasmic Reticulum,SR)内钙含量的影响。方法用100nmol·L-1NPY刺激Sprague-Dawley乳鼠心肌细胞24h,用荧光染料Fluo-4AM负载胞浆钙,并用咖啡因诱导的胞浆钙瞬变(Caffeine-induced Ca2+ transient,CCT)幅度来反映肌浆网内总钙负荷;用荧光染料Fluo-5NAM直接标记心肌细胞肌浆网内游离钙离子。所有钙影像均由LeicaSP2激光共聚焦显微镜记录。结果经100nmol·L-1NPY刺激24h后,心肌细胞肌浆网内游离钙含量明显低于对照组(p<0.01);咖啡因诱导下钙瞬变幅度也低于对照组。结论长时间的NPY刺激可导致肌浆网内钙含量减少。Objective To investigate the effect of neuropeptide Y （NPY） on calcium content in sarcoplasmic reticulum （SR） of rat cardiomyocytes.Methods Cardiomyocytes of neonatal Sprague-Dawley rats were incubated with NPY （100 nmol/L） for 24 h. Fluorescent assay with Fluo-4AM were used to detect Ca2＋ transients of cardiomyocytes and Fluo-5N AM were used to detect[Ca^2＋] in SR through laser scanning confocal microscope. The SR Ca^2＋ load was estimated by Caffeine-induced Ca^2＋ transient（CCT）.Results 24 hours after incubation, compared with control group, the concentration of free Ca^2 ＋ in SR（ [ Ca^2 ＋ ] ISR）was significantly decreased （p 〈 0. 05 ）, and the peak of CCT was attenuated.. Conclusions NPY lead to reduce the calcium content in sareoplasmic reticulum of rat eardiomyoeytes after a long period of stimulation.

关 键 词：神经肽Y 心肌细胞 肌浆网 钙瞬变 

分 类 号：R873.02[医药卫生—运动医学] R542.2[医药卫生—临床医学]