血红蛋白催化偶氮胂Ⅲ褪色测定过氧化氢  

Hemoglobin catalyzed Arsenazo Ⅲ fading reaction for the determination of hydrogen peroxide

作　　者：吴小华[1] 杨凯[1] 周炳江[1] 来海仙[1] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2009年第4期371-374,共4页Journal of Zhejiang Normal University:Natural Sciences

基　　金：浙江省自然科学基金资助项目(Y404012)

摘　　要：研究了牛血红蛋白（Hemoglob in,Hb）作为过氧化物模拟酶催化过氧化氢氧化偶氮胂Ⅲ（ArsenazoⅢ）褪色反应的催化特性和反应条件.结果表明：该催化反应体系在pH 4.0的HAc-NaAc缓冲溶液中、最大吸收波长536 nm处,褪色程度ΔA与H2O2的浓度之间具有线性关系,从而建立了测定H2O2的新方法.该方法的线性范围为1.03×10-5-2.58×10^-4mol·L^-1,表观摩尔吸光系数为2.46×10^3L·mol^-1·cm^-1.该方法用于消毒水中过氧化氢的测定,加标回收率为95.6%-106.1%.The catalystic activity of hemoglobin to oxidation of Arsenazo Ⅲ by hydrogen peroxide was studied.The catalystic characteristic and reaction condition of Hb-Arsenazo Ⅲ-H2O2 system were discussed.At pH 4.0 HAc-NaAc buffer solution,the system exhibited maximum absorption wavelength at 536 nm.The degree of fading（ΔA） was found linear to H2O2 concentration in the linear range of 1.03×10^-5～2.58×10^-4 mol·L^-1,and the apparentmolar absorptivity ε536 was 2.46×10^3 L·mo^l-1·cm^-1.The method was used in the determination of hydrogen peroxide in disinfector with satisfactory recoveries 95.6%～106.1%.

关 键 词：血红蛋白 过氧化氢 偶氮胂Ⅲ 催化光度法 

分 类 号：O652.6[理学—分析化学]