短波紫外线与交联淀粉碘对血浆中辛德比斯病毒灭活的研究  

STUDY ON INACTIVATION OF SINDBIS VIRUS IN PLASMA BY SHORT- WAVE ULTRAVIOLET RAYS AND CROSS-LINKED STARCH IODINE

作　　者：熊鸿燕[1,2] 刘育京[1,2] 涂瀛 

机构地区：[1]第三军医大学预防医学系 [2]军事医学科学院微生物流行病研究所

出　　处：《中国消毒学杂志》1998年第4期193-193,共1页Chinese Journal of Disinfection

摘　　要：对ＵＶＣ与ＣＳＩ分别对血浆中病毒灭活的效果和对血浆中ＦⅧ凝血活性的影响进行了观察。ＣＳＩ按１００ｍｇ／ｍｌ加入血浆，作用３０ｍｉｎ与６０ｍｉｎ，能使ＳＶ分别下降２５５与３６１个对数级，但血浆ＦⅧ凝血活性亦分别降至５７３３％和０；继续延长作用时间至１２０ｍｉｎ，灭活病毒的效果增加不明显。用剂量为７１７０～５７３６０Ｊ／ｍ２的ＵＶＣ照射血浆，可使ＳＶ下降４２６～６７１个对数级。照射剂量为７１７０Ｊ／ｍ２时，病毒灭活速度较快，ＦⅧ凝血活性也无损害，但超过此剂量后的病毒下降速度减缓，而对ＦⅧ凝血活性的损害明显加重。The efficacy of short-wave ultraviolet rays (UVC) and cross-linked starch iodine (CSI) in inactivating respectively Sindbis Virus in plasma and their influence on activity of Factor Ⅷ of the plasma were observed experimentally The results indicated that addition of CSI 100 mg/ml to plasma with contact time of 30, 60, 90 and 120 min could reduce Sindbis Virus (SV) in plasma by log 2 55, 3 61, 3 83 and 3 83 respectively, but the coagulation activity of plasma Factor Ⅷ decreased to 57 33% and 0 with 30 min and 60 min contact time respectively Irradiation of plasma with UVC at dosages of 7 170～57 360 J/m 2 could reduce SV by log 4 26～6 71 Irradiation with dosage of 7 170 J/m 2 could inactivate the virus rapidly and was essentially harmless to the activity of Factor Ⅷ, while the dosages higher than this showed decreased speed of inactivating the virus and had significant injurious effect on coagulating activity of Factor Ⅷ

关 键 词：短波紫外线 交联淀粉碘 血浆消毒 辛德比斯病毒 

分 类 号：R457.14[医药卫生—治疗学] R187[医药卫生—临床医学]