MdMYB1转录因子调控红星苹果果实着色的计算机模拟分析及表达验证  

In silico Analysis and Expression Confirmation of the Regulation of Fruit Coloration by Transcriptional Factor MdMBY1 in Delicious Apple

作　　者：张华磊[1,2] 毛柯[1,2] 刘志[2,3] 谢兴斌[1,2] 冯晓明[1,2] 郝玉金[1,2] 

机构地区：[1]山东农业大学作物生物学国家重点实验室,山东泰安271018 [2]山东农业大学园艺科学与工程学院,山东泰安271018 [3]辽宁省果树科学研究所,辽宁熊岳115009

出　　处：《园艺学报》2009年第11期1581-1588,共8页Acta Horticulturae Sinica

基　　金：英东青年教师基金项目(111024);山东省科技攻关项目(2008GG10009011)

摘　　要：从‘红星’苹果(Malus domestica ‘Delicious’)果实中克隆获得了MdMYB1基因,通过生物信息学分析(in silico analysis)和活体细胞融合蛋白荧光观察,确定其编码蛋白定位于细胞核。同时,克隆了MdDFR和MdUFGT基因的启动子,模拟分析表明2个基因的启动子序列中含有MYB结合的顺式作用元件。为了探讨MdMYB1转录因子是否调控MdDFR和MdUFGT基因的表达,对不同光照条件下果皮花青素含量和基因表达进行了检测,结果表明光照能够诱导花青素积累,并迅速启动3个基因表达,且3个基因表现出高度相似的表达模式,表明MdMYB1转录因子可能直接调控MdDFR和MdUFGT基因的表达。In this study,MdMYB1 was cloned from Delicious apple. It was found that MdMYB1 protein was localized in nucleus indicated by in silico predication and GFP fusion protein fluorescence observation. In parallel,the promoter regions of MdDFT and MdUFGT were isolated and analyzed in silico. The result showed that both promoters contained cis-elements characterized as MYB binding sites. Furthermore,to examine if MdMYB1 triggers the expression of MdDFR and MdUFGT during fruit coloration,anthocyanin content,expression levels of MdMYB1,MdDFR and MdUFGT were investigated. The results demonstrated that light exposure markedly induced anthocyanin accumulation in fruit peel,along with a rapid initiation of gene expression. Interestingly,three genes were expressed in a highly similar pattern. In one word,our findings suggest that transcription factor MdMYB1 may directly regulate the expression of MdDFR and MdUFGT genes.

关 键 词：苹果 果实 着色基因 光诱导 表达分析 

分 类 号：S661.1[农业科学—果树学]