一氧化氮和过氧化氢对地被菊扦插生根的影响  

Effects of Nitric Oxide and Hydrogen Peroxide on Rooting of Ground-cover Chrysanthemum Cuttings

作　　者：廖伟彪[1,2] 张美玲[3,4] 吴永华 肖洪浪[2] 

机构地区：[1]甘肃农业大学农学院,甘肃省作物遗传改良与种质创新重点实验室,兰州730070 [2]中国科学院寒区旱区环境与工程研究所,兰州730000 [3]甘肃农业大学草业学院,兰州730000 [4]甘肃农业大学理学院,兰州730000 [5]兰州市园林科学研究所,兰州730070

出　　处：《园艺学报》2009年第11期1643-1650,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(40501076);中国科学院知识创新工程重要方向项目(KZCX3-SW-324);兰州市科技局科技攻关项目(07-1-04)

摘　　要：以地被菊(Dendranthema morifolium)品种‘北国之春’为试验材料,研究了一氧化氮(NO)和过氧化氢(H2O2)对其插穗生根的影响以及生根过程中插穗叶片超氧化物岐化酶(SOD)、过氧化物酶(POD)、多酚氧化酶(PPO)活性以及酚类物质含量的变化。结果表明,外源NO和H2O2可促进地被菊插穗生根以及根的生长,且表现出明显的浓度效应。促进生根最适硝普纳(SNP,NO供体)和H2O2的浓度分别为50和200μmol·L-1。同时,SNP和H2O2共同处理的地被菊插穗生根率显著高于SNP或H2O2单独处理,说明NO和H2O2在地被菊插穗生根过程中具有协同诱导效应。另外,相比对照,SNP、H2O2和两者共同处理插穗叶片的SOD和PPO活性显著提高。而SNP、H2O2和两者共同处理比对照分别具有更低的POD活性和酚类物质含量,且SNP+H2O2处理的POD活性和酚类物质含量低于SNP或H2O2单独处理。综合以上结果可知,50μmol·L-1SNP和200μmol·L-1H2O2处理可以提高插穗叶片SOD和PPO活性,降低POD的活性和酚类物质的含量,从而促进插穗生根和根的生长。Ground-cover chrysanthemum （Dendranthema morifolium ＇Beiguozhichun＇） was used to understand the effects of nitric oxide （NO） and hydrogen peroxide （H2O2） on rooting of plant cuttings and the changes of activities of superoxide dismutase （SOD）,peroxidase （POD） and polyphenol oxidase （PPO） and the total polyphenol content of cutting leaves.The results showed that the effects of exogenous H2O2 or NO on rooting of ground-cover chrysanthemum cuttings was dose dependent,with a maximal biological response at 200 μmol·L^-12O2 or 50 μmol·L^-1O donors SNP.At the same time,the rooting percentage of cuttings treated with both SNP and H2O2 was significantly higher than that of cuttings treated with SNP or H2O2 alone,which suggested that there might exist synergistic action between H2O2 and NO on mediating rooting.Additionally,compared with the control,SNP,H2O2 and SNP＋H2O2 treatments might significantly increase the activities of SOD and PPO of ground-cover chrysanthemum cuttings leaves.However,the activities of POD and the total polyphenol content were significantly higher in control than in other treatments during rooting.Moreover,lower POD activities and polyphenol content were attained in SNP＋H2O2 treatment than in SNP or H2O2 treatment alone.Together,these results indicated that 50 μmol·L^-1NP and 200 μmol·L^-12O2 treatments enhanced rooting and root growth synergistically and independently by stimulating the activities of SOD and PPO enzymes,and simultaneously by repressing POD activities and the production of polyphenol.

关 键 词：地被菊 一氧化氮(NO) 过氧化氢(H2O2) 插穗 生根 

分 类 号：S682.11[农业科学—观赏园艺]