四逆汤改善缺血心肌能量代谢的作用及其机制探讨  

Effects of Sini Decoction improving energy metabolism of ischemic myocardium and mechanisms of the effects

作　　者：吴伟康[1] 侯灿[1] 罗汉川[1] 程超[1] 谭红梅[1] 

机构地区：[1]中山医科大学病理生理教研室

出　　处：《中国病理生理杂志》1998年第6期634-636,共3页Chinese Journal of Pathophysiology

基　　金：国家中医药管理局重点课题

摘　　要：目的：探讨四逆汤改善缺血心肌能量代谢的作用及其机制。方法：垂体后叶素性小鼠心肌缺血模型，四逆汤（浓度１０３ｇ生药／Ｌ）灌胃，剂量０１ｍＬ／２０ｇｗｔ·ｄ－１，心肌超微结构用电镜观察分析，心肌ＡＴＰ含量用高效液相色谱法测定，乳酸浓度用常规生化方法测定。结果：与缺血组相比，四逆汤组缺血心肌线粒体损伤显著减轻（Ｐ＜００５），糖原消耗显著减少（Ｐ＜００５），乳酸浓度显著下降（Ｐ＜００５），心肌营养血流量显著上升（Ｐ＜００１），氧自由基浓度显著降低（Ｐ＜００１）。结论：四逆汤具有显著改善缺血心肌能量代谢的作用。Abstract AIM:To explore the effects of Sini Decoction(SD) on improving energy metabolism of ischemic myocardium and their possible mechanisms.METHODS:Pituitrin-induced myocardial ischemia model of mice was used,SD(1 gram crude drugs/mL) was fed at the dosage of 0.1mL/20g.wt·d -1 for three days. Myocardial ultrastructure was observed and analyzed through electron microscope.Following indexes were determined:adenosine triphosphate(ATP) contentof myocardium by high pressure liquid chromatography(HPLC),lactic acid concentration by routine biochemical method,nutritional blood flow(NBF) by 86 RB uptake test,oxygen free radical (OFR) concentration by electron spin resonance(ESR).RESULTS:SD alleviated ischemia injury of myocardial mitochondria, increased NBF and the content of ATP,decreased the concentration of lactic acid and OFR significantly.CONCLUTIONS:SD improved the energy metabolism of ischemia myocardium significantly,the above effects of SD might be related the increase of blood supply and removal of OFR from ischemia myocardium. MeSH Myocardium;Ischemia;Free radicals;Energy metabolism

关 键 词：氧自由基 能量代谢 心肌缺血 中医药疗法 

分 类 号：R259.422[医药卫生—中西医结合]